Selenium/Zinc-Enriched probiotics improve serum enzyme activity, antioxidant ability, inflammatory factors and related gene expression of Wistar rats inflated under heat stress
Selenium/Zinc-Enriched probiotics improve serum enzyme activity, antioxidant ability, inflammatory factors and related gene expression of Wistar rats inflated under heat stress
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DOI：
10.1016/j.lfs.2020.117464
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发表时间：
2020-05-01
期刊：
影响因子：
6.1
通讯作者：
Chen, Xingxiang
中科院分区：
文献类型：
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作者：
Malyar, Rahmani Mohammad;Li, Hu;Chen, Xingxiang
目的：探讨富硒富锌益生菌对高热应激大鼠生长性能、血清酶活性、抗氧化能力、炎症因子及基因表达的影响。主要方法：将60只6周龄雄性Wistar大鼠随机分为5组，每组12只，分别饲喂基础日粮(对照组)、基础日粮添加富锌益生菌(P)、富锌益生菌(锌)100 mg/L、富硒益生菌(SEP，0.3 mg/L)和富硒锌益生菌(SeZnP，0.3 mg/L)。0.3 mg+100 mg/L)。实验持续了30天。结果：热应激组血清天冬氨酸氨基转移酶、丙氨酸氨基转移酶、乳酸脱氢酶、肌酸激酶、尿素氮、肌酸和碱性磷酸酶水平显著高于磷、锌、硒、锌和硒锌锌组(P<；0.01)。而添加锌磷、硒锌磷和硒锌磷显著提高了谷胱甘肽含量、谷胱甘肽过氧化物酶和超氧化物歧化酶活性，降低了丙二醛含量(P<；0.05)。IL-2、IL-6、IL-8浓度显著升高，IL-10浓度显著降低(P<；0.05)。此外，GPX1和SOD1基因的表达显著增加，而COX-2、iNOS、HSP70和90mRNA的表达显著降低(P<；0.05)。意义：本研究提示，对于高温饲养的Wistar大鼠，补充硒锌磷可能是一种比锌磷或硒磷更好的营养补充方法。
Aims: The present study was carried out to investigate the influences of Selenium/Zinc-Enriched probiotics (SeZnP) on growth performance, serum enzyme activity, antioxidant capability, inflammatory factors and gene expression associated with Wistar rats inflated under high ambient thermal-stress.Main methods: Sixty male rates with six-weeks of age were randomly allocated into five groups (12 per group) and fed basal diet (Control), basal diet supplemented with probiotics (P), Zinc-Enriched probiotics (ZnP, 100 mg/L), Selenium-Enriched Probiotics (SeP, 0.3 mg/L) and Selenium/Zinc-Enriched probiotics (SeZnP, 0.3 mg + 100 mg/L). The experiment lasted 30 days. Blood and Tissues samples were taken to investigate serum enzyme activity, antioxidants capability and inflammatory factors by using of commercial kits and antioxidant, heat shock and inflammatory related molecules expressions were determined by qRT-PCR.Key findings: Data analysis revealed that thermal stress significantly increased the level of Aspartate-aminotransferase, Alanine-aminotransferase, Lactate-dehydrogenase, Creatine-kinase, blood urea nitrogen, Creatinine and Alkaline phosphatase compared to P, ZnP, SeP or SeZnP groups (P < 0.01). However, supplementation of ZnP, SeP, and SeZnP significantly enhanced glutathione content, glutathione-peroxidase & superoxide-dismutase activity, and decreased malondialdehyde content (P < 0.05). Moreover, the concentration of IL-2, IL-6 and IL-8 were significantly increased while IL-10 was significantly decreased (P < 0.05). Furthermore, the expression of GPx1 and SOD1 genes were significantly increased, but COX-2, iNOS, HSP70 and 90 mRNA levels were significantly decreased (P < 0.05). Finally, the highest influence of the mentioned parameters was observed in SeZnP supplemented group.Significance: Our study suggests that SeZnP supplementation serves as possible and best nutritive than ZnP or SeP for Wistar rats raising under high ambient temperature.