Complement fixation by pemphigus antibody. I. In vitro fixation to organ and tissue culture skin.
Complement fixation by pemphigus antibody. I. In vitro fixation to organ and tissue culture skin.
复制标题
天疱疮抗体的补体固定。
DOI：
10.1111/1523-1747.ep12261058
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Jordon,RE
中科院分区：
文献类型：
--
作者：
Kawana,S;Janson,M;Jordon,RE
虽然在天疱疮皮肤病变的细胞间质中经常检测到补体，但天疱疮抗体在体外固定补体的能力是有争议的。本研究的目的是进一步使用器官和组织培养方法测试天疱疮抗体的体外补体结合能力。将来自小鼠尾部的表皮细胞单层与天疱疮血清的纯化IgG级分一起孵育，然后与纯化Clq一起孵育。通过免疫荧光方法证明Clq以及IgG的结合。当用正常人血清代替纯化的Clq作为补体来源时，阳性C3和C4染色也是明显的。当用纯化的正常人血清IgG代替天疱疮IgG时，未观察到类似的免疫荧光染色。使用器官培养物获得天疱疮抗体体外补体结合的进一步证据。在纯化的天疱疮IgG中培养的正常人皮肤和猴食管粘膜的器官培养物显示IgG的细胞间物质结合。当用正常IgG代替天疱疮IgG时，未观察到结合。然后用补体（新鲜正常人血清）处理额外的器官培养切片，并通过体外补体染色进行检测。在与天疱疮IgG孵育的器官培养的皮肤和粘膜的细胞间质区域中注意到Clq、C4和C3的固定，但在与正常IgG孵育的器官培养的皮肤和粘膜中没有。天疱疮IgG器官培养皮肤切片用未标记的抗C3进行的既往处理阻断了阳性C3染色。这些结果表明，一些天疱疮抗体能够在体外激活补体。
Although complement is often detected in the intercellular substance of pemphigus skin lesions, the ability of pemphigus antibodies to fix complement in vitro is controversial. The purpose of this study was to test in vitro complement fixation abilities of pemphigus antibodies further using organ and tissue culture methods. Epidermal cell monolayers from mouse tail were incubated with the purified IgG fraction of pemphigus serum followed by purified Clq. Binding of Clq, as well as IgG was demonstrated by immunofluorescence methods. When purified Clq was replaced with normal human serum as a complement source, positive C3 and C4 staining were also evident. When purified IgG of normal human serum was used in place of pemphigus IgG, similar immunofluorescence staining was not observed. Further evidence for complement fixation in vitro by pemphigus antibodies was obtained using organ cultures. Organ culture of normal human skin and monkey esophageal mucosa cultured in purified pemphigus IgG showed intercellular substance binding of lgG. No binding was observed when normal IgG was substituted for pemphigus IgG. Additional organ culture sections were then treated with complement (fresh normal human serum) and tested by in vitro complement staining. Fixation of Clq, C4, and C3 was noted in intercellular substance areas of organ cultured skin and mucosa incubated with pemphigus IgG but not those incubated with normal IgG. Prior treatment of pemphigus IgG organ cultured skin sections with unlabeled anti-C3, blocked positive C3 staining. These results suggest that some pemphigus antibodies are capable of activating complement in vitro.