Discovery of a pimaricin analog JBIR-13, from Streptomyces bicolor NBRC 12746 as predicted by sequence analysis of type I polyketide synthase gene
Discovery of a pimaricin analog JBIR-13, from Streptomyces bicolor NBRC 12746 as predicted by sequence analysis of type I polyketide synthase gene
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DOI：
10.1007/s00253-008-1849-8
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发表时间：
2009-05-01
影响因子：
5
通讯作者：
Shin-ya, Kazuo
中科院分区：
文献类型：
--
作者：
Komaki, Hisayuki;Izumikawa, Miho;Shin-ya, Kazuo
对双色链霉菌NBRC 12746（T）的酮合酶结构域扩增子的序列分析揭示了先前未报道的I型聚酮酶（PKS-I）基因的存在。这些基因与参考PKS-1序列的聚类表明产生类似于匹马菌素的多烯化合物的可能性。因此，分析来自NBRC 12746（T）的培养样品的多烯化合物的产生。该菌株产生的抗真菌化合物显示四烯大环内酯的紫外吸收光谱。对纯化的化合物进行了光谱分析，确定了一种新的匹马菌素类似物JBIR-13（1）。因此，这项研究强烈建议，PKS-I基因的仔细分析，可以提供有价值的信息，在寻找新的生物活性化合物从系统发育分析预测的一类。
Sequence analysis of ketosynthase domain amplicons from Streptomyces bicolor NBRC 12746(T) revealed the presence of previously unreported type I polyketide synthases (PKS-I) genes. The clustering of these genes with the reference PKS-1 sequences suggested the possibility to produce a polyene compound similar to pimaricin. Thus, the cultured sample from NBRC 12746(T) was analyzed for the production of polyene compounds. The strain produced an antifungal compound which displayed the UV absorption spectrum of tetraene macrolides. The structure determination based on the spectroscopic analysis of the purified compound resulted in the identification of a novel pimaricin analog JBIR-13 (1). This study therefore strongly suggested that a careful analysis of PKS-I genes can provide valuable information in the search of novel bioactive compounds within a class predicted from phylogenetic analysis.