Further characterization of the actin-related protein Act3p/Arp4 of S. cerevisiae through mutational analysis
Further characterization of the actin-related protein Act3p/Arp4 of S. cerevisiae through mutational analysis
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通过突变分析进一步表征酿酒酵母肌动蛋白相关蛋白 Act3p/Arp4
DOI：
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发表时间：
2000
影响因子：
2.8
通讯作者：
S. Stefanov
中科院分区：
文献类型：
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作者：
S. Stefanov
在酵母ARP 4（ACT 3）基因的功能分析过程中，我们进一步表征了其蛋白产物Arp4p在细胞中的作用。我们报告说，虽然它是本地化的细胞核，Arp4p执行其功能独立的结合DNA直接。Arp4p的核心肌动蛋白结构（ATP结合口袋）和一个假定的核定位信号（NLS）的作用进行了测试，通过靶向诱变。结果表明，在正常条件下，ATP酶活性和NLS是该蛋白在细胞中的基本功能。此外，报告基因的使用证实Arp4p可能参与一些一般的转录调控机制。
In the course of functional analysis of the yeast ARP4 (ACT3) gene, we further characterized the role of its protein product Arp4p in the cell. We report that although it is localized in the nucleus, Arp4p performs its function independently of binding to DNA directly. The roles of the core actin structure (the ATP-binding pocket) and a putative Nuclear Localization Signal (NLS) of Arp4p were tested by targeted mutagenesis. The results suggest that under normal conditions, the ATPase activity and the NLS are dispensable for the essential function of this protein in the cell. Furthermore, the use of reporter genes confirmed that Arp4p could be involved in some general mechanism of transcriptional regulation.