Caveolin-1 and-3 dissociations from caveolae to cytosol in the heart during aging and after myocardial infarction in rat
Caveolin-1 and-3 dissociations from caveolae to cytosol in the heart during aging and after myocardial infarction in rat
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DOI：
10.1016/s0008-6363(02)00660-0
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发表时间：
2003-02-01
影响因子：
10.8
通讯作者：
Samuel, JL
中科院分区：
文献类型：
--
作者：
Ratajczak, P;Damy, T;Samuel, JL
目的：小窝蛋白是小窝的结构蛋白，调节包括一氧化氮(NO)产生在内的多种信号通路。在小窝蛋白家族中，小窝蛋白-1和小窝蛋白-3分别主要在内皮细胞和肌肉细胞中表达。在这项研究中，我们研究了(I)在大鼠心脏衰老和衰竭过程中小窝蛋白丰度和/或分布的变化，以及(Ii)这一过程是否会影响一氧化氮合酶(NOS)的活性。方法：应用免疫组织化学和免疫印迹方法，分析小窝蛋白在成年(Ad)、老年(S-Sh)和心梗(S-MI)心肌组织中的表达和分布。免疫沉淀法检测NOS3/小窝蛋白-1的相互作用。结果：在显微镜水平上，小窝蛋白-1在内皮细胞中的分布在两组之间没有变化。相反，S心肌梗死大鼠心肌细胞肌膜小窝蛋白-3的典型分布发生了显著的变化，导致了整个间隔的异质性模式。无论哪一组，小窝蛋白-1和-3的总丰度都保持稳定。在去小窝(Triton X-100可溶)组中，小窝蛋白-1a和-3水平随着年龄的增长而增加(+20%，+104%，P
Objective: Caveolins, the structural proteins of caveolae, modulate numerous signaling pathways including Nitric Oxide (NO) production. Among the caveolin family, caveolin-1 and -3 are mainly expressed in endothelial and muscle cells, respectively. In this study, we investigate whether (i) changes in caveolin abundance and/or distribution occur during cardiac aging and failure in rat, and (ii) the process could influence NO synthase (NOS) activity. Methods: Using immunohistolabelling and Western blot approaches, expression and distribution of caveolins were analysed in adult (Ad), senescent (S-Sh) and myocardial in farction-induced failing (S-MI) hearts. NOS3/caveolin-1 interactions were evaluated by immunoprecipitation assays. Results: At the microscope level, caveolin-1 distribution in the endothelial cells was unchanged between the groups. Conversely the typical distribution of caveolin-3 in myocyte sarcolemma was dramatically altered in S-MI rats, resulting in a heterogeneous pattern throughout the septum. Total abundance of caveolin-1 and -3 remained stable whatever the group. In the fractions free of caveolae (Triton X-100 soluble), the levels of caveolin-la and -3 increased with aging (+20%, and +104%, P