Comprehensive Transcriptome Analyses Reveal Differential Gene Expression Profiles of Camellia sinensis Axillary Buds at Para-, Endo-, Ecodormancy, and Bud Flush Stages.
Comprehensive Transcriptome Analyses Reveal Differential Gene Expression Profiles of Camellia sinensis Axillary Buds at Para-, Endo-, Ecodormancy, and Bud Flush Stages.
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综合转录组分析揭示了茶树腋芽在旁休眠、内休眠、生态休眠和芽盛期的差异基因表达谱
DOI：
10.3389/fpls.2017.00553
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发表时间：
2017
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Hao X;Yang Y;Yue C;Wang L;Horvath DP;Wang X
茶树是世界上为数不多的收获叶片的木本植物之一，也是为数不多的具有休眠特性的非针叶万年青树种之一，冬眠性是茶树度过寒冷冬季的重要生物学特性，也影响着茶树的经济产量。为揭示茶树冬季芽休眠的调控机制，采用RNA-Seq技术分析了茶树腋芽在准休眠期、内休眠期、外休眠期和盛芽期的基因表达谱。在不同的测量条件下，总共鉴定了16，125个差异表达基因（DEG）。对从每个休眠过渡中鉴定的DEG进行基因集富集分析。丰富的基因本体论术语、基因组和转录因子主要与表观遗传机制、植物激素信号通路和胼胝体相关的细胞通讯调控有关。此外，差异表达的转录因子，以及染色质和phytochrome相关基因进行了鉴定。GI-、CAL-、SVP-、PHYB-、SFR 6-、LHY-、ZTL-、PIF 4/6-、ABI 4-、EIN 3-、ETR 1-、CCA 1-、PIN 3-、CDK-和CO相关基因集被富集。通过序列同源性分析，总结了白杨和茶树中表达差异显著的关键基因。茶树休眠调控的主要分子途径与白杨有一定的一致性，但基因表达模式存在差异。该研究提供了茶树越冬芽不同休眠期的转录组图谱，对提高对茶树芽休眠的认识具有重要意义。
Winter dormancy is an important biological feature for tea plant to survive cold winters, and it also affects the economic output of tea plant, one of the few woody plants in the world whose leaves are harvested and one of the few non-conifer evergreen species with characterized dormancies. To discover the bud dormancy regulation mechanism of tea plant in winter, we analyzed the global gene expression profiles of axillary buds at the paradormancy, endodormancy, ecodormancy, and bud flush stages by RNA-Seq analysis. In total, 16,125 differentially expressed genes (DEGs) were identified among the different measured conditions. Gene set enrichment analysis was performed on the DEGs identified from each dormancy transition. Enriched gene ontology terms, gene sets and transcription factors were mainly associated with epigenetic mechanisms, phytohormone signaling pathways, and callose-related cellular communication regulation. Furthermore, differentially expressed transcription factors as well as chromatin- and phytohormone-associated genes were identified. GI-, CAL-, SVP-, PHYB-, SFR6-, LHY-, ZTL-, PIF4/6-, ABI4-, EIN3-, ETR1-, CCA1-, PIN3-, CDK-, and CO-related gene sets were enriched. Based on sequence homology analysis, we summarized the key genes with significant expression differences in poplar and tea plant. The major molecular pathways involved in tea plant dormancy regulation are consistent with those of poplar to a certain extent; however, the gene expression patterns varied. This study provides the global transcriptome profiles of overwintering buds at different dormancy stages and is meaningful for improving the understanding of bud dormancy in tea plant.