Engineering microbial hosts for production of bacterial natural products.
Engineering microbial hosts for production of bacterial natural products.
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DOI：
10.1039/c6np00017g
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发表时间：
2016-08-27
影响因子：
11.9
通讯作者：
Zhao H
中科院分区：
文献类型：
--
作者：
Zhang MM;Wang Y;Ang EL;Zhao H
微生物发酵提供了一个有吸引力的替代化学合成生产结构复杂的天然产物。然而，在大多数情况下，生产滴度很低，需要改进以进行化合物表征和/或商业生产。由于功能基因组学和基因工程技术的进步，微生物宿主可以被设计成过量生产所需的天然产物，大大加快了传统上耗时的菌株改良过程。本文综述了用于生产细菌天然产物的原生和异源微生物宿主工程的最新进展和挑战，重点介绍了菌株改良的遗传工具和策略。特别强调的是放线菌的生物活性次生代谢物。选择主机系统的考虑因素也将在本综述中讨论。
Microbial fermentation provides as an attractive alternative to chemical synthesis for the production of structurally complex natural products. In most cases, however, production titers are low and need to be improved for compound characterization and/or commercial production. Owing to advances in functional genomics and genetic engineering technologies, microbial hosts can be engineered to overproduce a desired natural product, greatly accelerating the traditionally time-consuming strain improvement process. This review covers recent developments and challenges in the engineering of native and heterologous microbial hosts for production of bacterial natural products, focusing on the genetic tools and strategies for strain improvement. Special emphasis is placed on bioactive secondary metabolites from actinomycetes. The considerations for the choice of host systems will also be discussed in this review.