Virus-inspired approach to nonviral gene delivery vehicles.
Virus-inspired approach to nonviral gene delivery vehicles.
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DOI：
10.1021/bm900370p
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发表时间：
2009-08-10
影响因子：
6.2
通讯作者：
Thayumanavan S
中科院分区：
文献类型：
--
作者：
Roy R;Jerry DJ;Thayumanavan S
负责HIV-TAT蛋白易位的基本达特肽已经与各种人工纳米级材料缀合，以将它们运输穿过细胞膜。然而，尝试使用TAT-肽易位基因遇到了有限的成功。我们假设肽的阳离子性质不允许在复合物的表面上展示这些肽。为了规避这个潜在的问题，我们已经开发了一种新的分子设计策略，其中TAT-肽可以有效地显示在复合物的表面上，从而增强基因表达。
The basic TAT peptide, responsible for translocation of the HIV-TAT protein, has been conjugated to a variety of artificial nanoscopic materials to transport them across the cellular membrane. However, attempts to translocate genes using the TAT-peptide had met with limited success. We hypothesized that the cationic nature of the peptide does not allow for displaying these peptides on the surface of the polyplex. To circumvent this potential issue, we have developed a new molecular design strategy where the TAT-peptide can be effectively displayed on the surface of the polyplex, thus enhancing gene expression.