Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography
Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography
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DOI：
10.1016/j.chroma.2004.02.080
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发表时间：
2004-05-21
影响因子：
4.1
通讯作者：
Wang, YT
中科院分区：
文献类型：
--
作者：
Li, SP;Li, P;Wang, YT
建立了同时测定虫草中麦角甾醇、核苷及其碱基含量的方法。采用加压液体萃取法（PLE）对样品进行萃取。实验变量，如溶剂，温度，静态萃取时间和周期，对PLE效率的影响进行了研究。结果表明，溶剂和温度对PLE的提取效率有很大的影响。使用Zorbax NH 2分析柱（250 x 4.6 mm i.d.，5 μ m）。样品的自动制备允许非常快速的分析，这是常规目的的重要目标。(C)2004 Elsevier B.V.保留所有权利。
A simple method is described for the simultaneous determination of ergosterol, nucleosides and their bases in Cordyceps. The samples were extracted by using pressurised liquid extraction (PLE). The effects of experimental variables, such as solvent, temperature, static extraction time and cycles, on PLE efficiency have been studied. The results showed a strong influence of the solvent and temperature on extraction efficiency of PLE. The determination was achieved by high-performance liquid chromatography (HPLC) using a Zorbax NH2 analytical column (250 x 4.6 mm i.d., 5 mum) with diode-array detector (DAD). The automated preparation of the sample permits a very fast analysis which is an important goal for routine purpose. (C) 2004 Elsevier B.V. All rights reserved.