Role of PI3K/Akt signaling in the protective effect of magnesium sulfate against ischemia-perfusion injury of small intestine in rats
Role of PI3K/Akt signaling in the protective effect of magnesium sulfate against ischemia-perfusion injury of small intestine in rats
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DOI：
10.3760/cma.j.issn.0366-6999.2010.11.018
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发表时间：
2010-06-05
影响因子：
6.1
通讯作者：
Tan Zhi
中科院分区：
文献类型：
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作者：
Chen Shi-da;Chen Ya-bin;Tan Zhi
背景硫酸镁对SD大鼠小肠缺血再灌注损伤的保护作用已被我们前期的研究所证实。然而，其确切机制尚不清楚。本研究旨在探讨PI 3 K/Akt信号通路在硫酸镁对SD大鼠小肠缺血再灌注损伤保护作用中的作用。将SD大鼠随机分为假手术组、缺血再灌注组、硫酸镁组和硫酸镁+LY 294002（PI 3 K抑制剂）组。观察肠黏膜病理变化，测定血浆二胺氧化酶（DAO）活性、丙二醛（MDA）含量及肠黏膜细胞凋亡率，并进行比较。结果肠黏膜组织病理改变较肠黏膜组织明显（Chiu's评分高，P
Background The protective effects of magnesium sulfate against ischemia-reperfusion injury of the small intestine in Sprague-Dawley (SD) rats have been confirmed in our previous research. However, its exact mechanism is unclear. This study was to evaluate the role of PI3K/Akt signal pathway in the protective effect of magnesium sulfate against ischemia-reperfusion injury of the small intestine in SD rats.Methods Rat model of intestinal ischemia-reperfusion injury was used. The SD rats were divided into four groups randomly: sham operation group, ischemia-reperfusion group, magnesium sulfate group and magnesium sulfate plus LY294002 (an inhibitor of PI3K) group. The pathological changes of intestinal mucosa were examined; the activity of diamine oxidase (DAO) in plasma, the plasma contents of malondialdehyde (MDA), and apoptosis rate of the intestinal mucosal cells were determined and compared. The expression of p-Akt was detected by Western blotting.Results There were more evident pathological changes of the intestinal mucosa (higher Chiu's score, P