Housekeeping genes as internal standards: use and limits
Housekeeping genes as internal standards: use and limits
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DOI：
10.1016/s0168-1656(99)00163-7
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发表时间：
1999-10-08
影响因子：
4.1
通讯作者：
Heinen, E
中科院分区：
文献类型：
--
作者：
Thellin, O;Zorzi, W;Heinen, E
定量研究通常在生物医学研究中实现，以比较不同实验或临床条件下的RNA表达。这些定量是通过与3-磷酸甘油醛脱氢酶(G3PDH)、白蛋白、肌动蛋白、微管蛋白、亲环素、低毒磷酸核糖转移酶(HRPT)、L32等看家基因转录本的表达进行比较来进行的。28S和18S rRNA也被用作内标。在这篇文章中，回顾了常用的内部标准可以根据不同的因素而定量地变化。用三个实验例子说明了可能的变化。然后为每个样品提出了优选的内标物类型，并讨论了选择内标物的一般程序和管理其使用的方法。(C)1999 Elsevier Science B.V.保留所有权利。
Quantitative studies are commonly realised in the biomedical research to compare RNA expression in different experimental or clinical conditions. These quantifications are performed through their comparison to the expression of the housekeeping gene transcripts like glyceraldehyde-3-phosphate dehydrogenase (G3PDH), albumin, actins, tubulins, cyclophilin, hypoxantine phosphoribosyltransferase (HRPT), L32. 28S and 18S rRNAs are also used as internal standards. In this paper, it is recalled that the commonly used internal standards can quantitatively vary in response to various factors. Possible variations are illustrated using three experimental examples. Preferred types of internal standards are then proposed for each of these samples and thereafter the general procedure concerning the choice of an internal standard and the way to manage its used are discussed. (C) 1999 Elsevier Science B.V. All rights reserved.