Rapid serologic diagnosis of dengue virus infection using a commercial capture ELISA that distinguishes primary and secondary infections
Rapid serologic diagnosis of dengue virus infection using a commercial capture ELISA that distinguishes primary and secondary infections
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DOI：
10.4269/ajtmh.1999.60.693
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发表时间：
1999-04-01
影响因子：
3.3
通讯作者：
Devine, PL
中科院分区：
文献类型：
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作者：
Vaughn, DW;Nisalak, A;Devine, PL
登革热感染期间产生的特异性IgM和IgG抗体的商业捕获ELISA（PanBio Dengue Due）使用在出院时收集的血清与已建立的ELISA和血凝抑制（HAI）测定相比显示出优异的灵敏度（99%，n = 78）。此外，ELISA能够使用入院时收集的血清诊断79%的登革热病例。ELISA对26例非黄病毒感染者的配对血清也显示出高特异性（92%），而45%的日本脑炎患者（n = 20）显示IgG升高而IgM不升高。IgG捕获ELISA法与HAI法具有良好的相关性（r = 0.83，P < 0.0001），IgG水平可用于区分原发性感染和继发性感染，原发性感染的正确分类率为100%，继发性感染的正确分类率为96%。这种ELISA应该被证明在登革热感染的临床诊断中是有用的。
A commercial capture ELISA for specific IgM and IgG antibodies produced during dengue infection (PanBio Dengue Due) showed excellent sensitivity (99%, n = 78) using sera collected at hospital discharge compared with established ELISA and hemagglutination inhibition (HAI) assays. Furthermore, the ELISA was able to diagnose 79% of the dengue cases using sera collected at hospital admission. The ELISA also showed high specificity (92%) in paired sera from patients without flavivirus infection (n = 26), although 45% of the patients with Japanese encephalitis (n = 20) showed elevation of IgG but not IgM. The IgG capture ELISA showed good correlation with the HAI assay (r = 0.83, P < 0.0001), and IgG levels could be used to distinguish between primary and secondary infection, with 100% of primary infections and 96% of secondary infections being correctly classified. This ELISA should prove useful in the clinical diagnosis of dengue infections.