GROUP-A STREPTOCOCCI EFFICIENTLY INVADE HUMAN RESPIRATORY EPITHELIAL-CELLS
GROUP-A STREPTOCOCCI EFFICIENTLY INVADE HUMAN RESPIRATORY EPITHELIAL-CELLS
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DOI：
10.1073/pnas.91.25.12115
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发表时间：
1994-12-06
影响因子：
11.1
通讯作者：
CLEARY, PP
中科院分区：
文献类型：
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作者：
LAPENTA, D;RUBENS, C;CLEARY, PP
虽然A组链球菌感染是一种细胞外粘膜致病模式，但这些细菌可能与导致败血症和休克的高侵袭性感染有关。在过去的6年里，该物种重新获得了作为败血症重要原因的声誉，并激起了人们对某些菌株比其他菌株更能突破粘膜屏障进入血液的机制的兴趣。根据胞内链球菌对青霉素和庆大霉素的耐药性，建立了内化试验。实验表明，与对数相相反，细菌可以有效地内化并在培养的人类细胞中存活。电子显微镜证实链球菌存在于细胞内的空泡中。不同菌株的链球菌在内化能力上存在显著差异。从血液感染中分离出的两种M1型链球菌的内化频率与沙门氏菌和单核细胞增生性李斯特菌的报告频率相同，并高于咽炎病例中克隆变体的频率。
Although infection by group A streptococci is a model of extracellular mucosal pathogenesis, these organisms can be associated with highly invasive infections resulting in sepsis and shock. Over the last 6 yr this species has renewed its reputation as a significant cause of sepsis and has piqued interest in the mechanism by which some strains are better able to breach mucosal barriers to gain access to the bloodstream than are others. An internalization assay was developed on the basis of resistance of intracellular streptococci to penicillin and gentamicin. Experiments showed that stationary-phase, as opposed to logarithmic-phase, bacteria are efficiently internalized and can persist in cultured human cells. Electron microscopy confirmed that streptococci were contained within intracellular vacuoles. Various strains of streptococci revealed significant differences in their capacity to be internalized. Two type M1 streptococci isolated from blood infections were internalized at frequencies equal to those reported for Salmonella and Listeria monocytogenes and greater than the frequency of a clonal variant from a case of pharyngitis.