Comparison of the protein composition of Erysipelothrix rhusiopathiae strains of subtype 1A isolated from ducks and pigs.
Comparison of the protein composition of Erysipelothrix rhusiopathiae strains of subtype 1A isolated from ducks and pigs.
复制标题
鸭和猪猪红斑丹毒丝菌 1A 亚型菌株蛋白质组成比较。
DOI：
--
复制
发表时间：
1998
影响因子：
0.9
通讯作者：
G. Szita
中科院分区：
文献类型：
--
作者：
S. Bernáth;G. Morovján;V. Sztojkov;G. Szita
用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对从鸭和猪体内分离的猪丹毒丝菌进行了检测，并通过计算机方法对结果进行了评价，以根据分子量比较菌株的蛋白质组成。计算Pearson相关系数，以确定菌株间的相似性。来自同一鸭群的鸭株之间的相似度为980-991，而从鸭和猪分离的菌株在0 - 1000的尺度上在836 - 991之间变化。该方法给出了高度重复性的结果，并可用于流行病学调查。它的应用可能为E.因此，我们需要对Rhusiopathiae菌株进行研究，以补充先前为此目的推荐的方法的结果，并提高流行病学调查的效率。
Erysipelothrix rhusiopathiae strains isolated from duck carcasses and pigs were examined by sodium dodecyl sulphate--polyacrylamide gel electrophoresis (SDS-PAGE) and the results were evaluated by a computerised method in order to compare the strains for protein composition according to molecular mass. To characterise the similarity of the strains, Pearson's correlation coefficient was calculated. The degree of similarity between duck strains originating from the same flock was 980-991, while that of strains isolated from ducks and pigs varied between 836 and 991 on a scale of 0 to 1000. The method gives highly reproducible results and can be utilised in epidemiological investigations. Its use may provide new data on E. rhusiopathiae strains, complementing the results of methods recommended for this purpose earlier and increasing the efficiency of epidemiological investigations.