A Toxin Isolated from Sarcocystis fayeri in Raw Horsemeat May Be Responsible for Food Poisoning
A Toxin Isolated from Sarcocystis fayeri in Raw Horsemeat May Be Responsible for Food Poisoning
复制标题
DOI：
10.4315/0362-028x.jfp-13-351
复制
发表时间：
2014-05-01
影响因子：
2
通讯作者：
Sugita-Konishi, Yoshiko
中科院分区：
文献类型：
--
作者：
Kamata, Yoichi;Saito, Morihiro;Sugita-Konishi, Yoshiko
在日本，有食用生马肉后发生食物中毒的报道。潜伏期短的腹泻是这类食物中毒的常见症状。根据形态学和遗传学评价以及对犬进行包囊实验性喂养（导致孢子囊排泄）的结果，已将患者摄入的马肉中发现的包囊鉴定为Sarcocystis fayeri。含有包囊的马肉提取物在家兔回肠袢试验中产生阳性肠毒性反应。静脉注射从囊肿中分离的15 kDa蛋白质诱导兔腹泻和致死毒性，并且该蛋白质在回肠环试验中产生肠毒性，含有囊肿的马肉提取物也产生肠毒性。15 kDa蛋白的部分氨基酸序列与弓形虫和柔嫩艾美耳球虫肌动蛋白解聚因子同源。这些结果表明，S. fayeri是一种毒素，食用寄生马肉后会导致食物中毒。
Food poisoning has been reported after the consumption of raw horsemeat in Japan. Diarrhea with a short incubation period is a common symptom in such cases of food poisoning. Cysts found in horsemeat ingested by patients have been identified as Sarcocystis fayeri based on morphological and genetic evaluation and findings from experimental feeding of cysts to dogs, which resulted in the excretion of sporocysts. The extracts of the horsemeat containing the cysts produced a positive enterotoxic response in the rabbit ileal loop test. Intravenous injection of a 15-kDa protein isolated from the cysts induced diarrhea and lethal toxicity in rabbits, and the protein produced enterotoxicity in the ileal loop test as did the extracts of the horsemeat containing the cysts. The partial amino acid sequence of the 15-kDa protein was homologous to the actin-depolymerizing factor of Toxoplasma gondii and Eimeria tenella. These findings indicate that the 15-kDa protein of S. fayeri is a toxin that causes food poisoning after consumption of parasitized horsemeat.