Determination of analyte concentration using the residual solvent resonance in 1H NMR spectroscopy
Determination of analyte concentration using the residual solvent resonance in 1H NMR spectroscopy
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DOI：
10.1021/np8000046
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发表时间：
2008-05-01
影响因子：
5.1
通讯作者：
Quinn, Ronald J.
中科院分区：
文献类型：
--
作者：
Pierens, Gregory K.;Carroll, Anthony R.;Quinn, Ronald J.
使用来自DMSO-d（6）的残留质子信号的NMR方案（即，DMSO-d（5））测定NMR样品中分析物的浓度。该技术提供了一种替代方法，用于确定溶液中化合物的摩尔浓度，而无需事先了解其分子量。当要分析亚毫克量的化合物时，该方法特别有用，并且适用于各种不同的研究领域，例如化合物管理、天然产物、组合和药物化学。
An NMR protocol that uses the residual proton signal from DMSO-d(6) (i.e., DMSO-d(5)) to determine the concentration of an analyte in a NMR sample was developed. This technique provides an alternative method for determining the molar concentration of compounds in solution without prior knowledge of their molecular weight. The method is particularly useful when submilligram quantities of compound are to be analyzed and is applicable to a variety of different research areas such as compound management, and natural product, combinatorial, and medicinal chemistry.