Atrial natriuretic factor elicits an endothelium-independent relaxation and activates particulate guanylate cyclase in vascular smooth muscle.
Atrial natriuretic factor elicits an endothelium-independent relaxation and activates particulate guanylate cyclase in vascular smooth muscle.
复制标题
心房钠尿因子引起内皮依赖性松弛并激活血管平滑肌中的颗粒鸟苷酸环化酶。
DOI：
10.1073/pnas.81.23.7661
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发表时间：
1984
影响因子：
11.1
通讯作者：
Rapoport,RM
中科院分区：
文献类型：
--
作者：
Winquist,RJ;Faison,EP;Waldman,SA;Schwartz,K;Murad,F;Rapoport,RM
心房利钠因子（ANF） 26个氨基酸合成肽片段放松内皮完整或功能破坏的离体兔主动脉段。这种松弛与cGMP水平的升高有短暂的相关性，而cAMP水平没有变化。在无钙缓冲液或cGMP磷酸二酯酶抑制剂M&B 22,948预处理的主动脉段中，也观察到anf诱导的cGMP升高。硝普钠也得到了类似的定性结果。ANF选择性地激活颗粒鸟苷酸环化酶，对酶的可溶性形式没有影响。因此，ANF的直接（内皮独立的）血管扩张作用可能是通过增加组织中cGMP水平介导的。ANF似乎通过激活颗粒鸟苷酸环化酶来增加血管cGMP水平。
A 26 amino acid synthetic peptide fragment of atrial natriuretic factor (ANF) relaxed isolated rabbit aortic segments in which the endothelium was either intact or functionally destroyed. The relaxations were temporally associated with increases in levels of cGMP with no change in the levels of cAMP. The ANF-induced increases in cGMP were also observed in aortic segments pretreated with calcium-free buffer or the cGMP phosphodiesterase inhibitor M&B 22,948. Qualitatively similar results were obtained for sodium nitroprusside. ANF selectively activated particulate guanylate cyclase, having no effect on the soluble form of the enzyme. Thus, the direct (endothelium-independent) vasodilator effect of ANF may be mediated via increased tissue levels of cGMP. ANF appears to increase vascular cGMP levels by activation of particulate guanylate cyclase.