Resistance of Lactobacillus plantarum KCTC 3099 from Kimchi to oxidative stress
Resistance of Lactobacillus plantarum KCTC 3099 from Kimchi to oxidative stress
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DOI：
10.1089/jmf.2005.8.299
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发表时间：
2005-09-01
影响因子：
2.4
通讯作者：
Park, KY
中科院分区：
文献类型：
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作者：
Lee, J;Hwang, KT;Park, KY
从韩国发酵食品泡菜中分离出两株乳酸菌，通过测定其对活性氧的抵抗力来评价其抗氧化能力，并与阳性对照鼠李糖乳杆菌的抗氧化能力进行比较。植物乳杆菌KCTC3099的完整细胞和无细胞提取物在抑制脂质过氧化方面表现出较高的抗氧化活性，抑制率分别为38.6%和48.5%。为了评估这两株乳酸菌对ROS的抗性，我们测试了它们在1 mM过氧化氢、0.4 mM羟基自由基和10 mM百草枯诱导的超氧阴离子存在下的存活率。植物乳杆菌KCTC 3099在1 mM的过氧化氢和0.4 mM的羟基自由基存在下，即使在8小时后仍能存活。更有甚者。植物乳杆菌KCTC3099的存活不受百草枯产生的超氧阴离子的影响，表明其对超氧阴离子具有抗性。测定超氧化物歧化酶(SOD)活性和金属离子螯合活性，以明确其抗氧化机制。植物乳杆菌KCTC 3099对Fe2+、Cu2+、C和金属离子均有较高的螯合活性，分别为13.6ppm和23.9ppm。这些结果表明，植物乳杆菌KCTC3099的抗氧化能力明显是由金属离子的络合作用引起的，而不是由超氧化物歧化酶的活性引起的。
The antioxidative capacity of two lactic acid bacteria isolated from Kimchi, a Korean fermented food, was evaluated by measuring the resistance to reactive oxygen species (ROS) and compared with that of Lactobacillits rhamnosus GG as a positive control. Both intact cells and cell-free extracts of Lactobacillus plantartan KCTC 3099 exhibited higher antioxidative activity in inhibiting lipid peroxidation among the strains evaluated with an inhibitory level of 38.6% and 48.5%, respectively. To evaluate the resistance of the two lactic acid bacteria to ROS, we tested their survival in the presence of I mM hydrogen peroxide, 0.4 mM hydroxyl radicals, and superoxide anions induced by 10 mM paraquat. L. plantarum KCTC 3099 was viable even after 8 hours in the presence of both I mM hydrogen peroxide and 0.4 mM hydroxyl radicals. Moreover. the survival of L. plantarum KCTC 3099 was not affected by superoxide anions generated by using paraquat, indicating that it has resistance to superoxide anions. To define the antioxidative mechanism, superoxide dismutase (SOD) and metal ion chelating activities were determined. L. plantarton KCTC 3099 presented little SOD activity, but had the higher level of chelatinc, activity for both Fe2+ CU2+ C and metal ions at 13.6 ppm and 23.9 ppm, respectively. These results suggested that the antioxidative capacity of L. plantarton KCTC 3099 is apparently caused by chelating metal ions rather than by SOD activation.