Use of a rapid, single-round, multiplex PCR to detect malarial parasites and identify the species present
Use of a rapid, single-round, multiplex PCR to detect malarial parasites and identify the species present
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DOI：
10.1179/000349803125002977
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发表时间：
2003-03-01
影响因子：
--
通讯作者：
Saldanha, J
中科院分区：
文献类型：
--
作者：
Padley, D;Moody, AH;Saldanha, J
一种基于单轮多重PCR的新型快速检测方法可用于检测人血液中的恶性疟原虫、间日疟原虫、三日疟原虫或卵圆疟原虫。针对寄生虫保守的18S小亚基RNA基因的PCR不仅允许检测疟疾感染，而且还允许鉴定存在的每个疟原虫物种，即使在混合感染的情况下。
A new, rapid assay, based on a single-round, multiplex PCR, can be used to detect Plasmodium falciparum, P. vivax, P. malariae or P. ovale in human blood. The PCR, which targets the conserved 18S small-subunit RNA genes of the parasites, not only permits a malarial infection to be detected but also allows each Plasmodium species present to be identified, even in cases of mixed infection.