Magnetic particles in the liver: a probe for intracellular movement
Magnetic particles in the liver: a probe for intracellular movement
复制标题
肝脏中的磁性粒子：细胞内运动的探针
作者：
P. Gehr;J. Brain;S. B. Bloom;P. Valberg
以前的研究使用磁性颗粒来估计体外培养1-4中细胞质的粘度。在这里，我们描述了如何使用磁性Fe_2O_3颗粒来非侵入性地估计完整动物肝巨噬细胞中细胞器的运动。我们报道，当这些颗粒被静脉注射时，大多数被肝巨噬细胞吞噬(图1)5。当外部磁场施加到兔身上时，这些颗粒被磁化并排列。去除磁场后，这些粒子共同产生一个剩余磁场，可以在人体表面测量到。由于粒子旋转(松弛)6，7，磁场随时间减小。由于粒子包含在吞噬小体或次级溶酶体中，我们得出结论，这些细胞器的运动是粒子旋转和松弛的原因。
Previous studies have used magnetic particles to estimate the viscosity of cell cytoplasm in vitro1–4. Here we describe how magnetic Fe2O3 particles can be used to estimate non-invasively the motion of organelles in hepatic macrophages in intact animals. We report that when these particles are injected intravenously (i.v.), most are phagocytosed by hepatic macrophages (Fig. 1)5. When an external magnetic field is applied to the rabbit, these particles become magnetized and aligned. After removal of the field, the particles collectively produce a remanent magnetic field which can be measured at the body surface. This field decreases with time due to particle rotation (relaxation)6,7. As the particles are contained in phagosomes or secondary lysosomes, we conclude that motions of these organelles are responsible for the particle rotation and relaxation.