Transgenic RNA interference in mice
Transgenic RNA interference in mice
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DOI：
10.1152/physiol.00002.2007
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发表时间：
2007-06-01
期刊：
影响因子：
8.4
通讯作者：
Zhang, Pumin
中科院分区：
文献类型：
--
作者：
Gao, Xue;Zhang, Pumin
The discovery that small interfering RNA duplexes (siRNA) can silence gene expression in mammalian cells has revolutionized biomedical research. The most successful application of the discovery has been to study gene function in cultured human or mouse cells. However, the knockdown effect of siRNA is only transient. To achieve a more sustained gene-silencing effect, shRNA (small hairpin RNA interference (RNAi) expressed from a vector is preferred. An additional benefit of shRNA-expressing vectors by transgenic technology. Transgenic RNAi not only allows the study of biological processes not present in cultured cells but also offers chronic therapeutic potentials. In this review we will summarize the developments in the generation of transgenic RNAi mice.
