The role of zinc in the binding of killer cell Ig-like receptors to class I MHC proteins
The role of zinc in the binding of killer cell Ig-like receptors to class I MHC proteins
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DOI：
10.1073/pnas.041618298
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发表时间：
2001-02-13
影响因子：
11.1
通讯作者：
Reyburn, HT
中科院分区：
文献类型：
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作者：
Valés-Gómez, M;Erskine, RA;Reyburn, HT
杀伤细胞免疫球蛋白样受体（KIR）与其I类MHC配体的结合以前被证明具有极快的结合和解离速率常数。在更详细地研究受体-配体结合的生物化学的实验期间，观察到相互作用的动力学参数在Zn 2+而不是其他二价阳离子的存在下显著改变。这种现象的基础是Zn 2+诱导的KIR分子的多聚化，如BIAcore、分析超离心和化学交联实验所证明的。KIR的Zn 2+依赖性多聚化可能是KIR和HLA-C分子簇形成的关键，即在NK细胞和靶细胞之间接触部位观察到的“自然杀伤（NK）细胞免疫突触”。
The binding of killer cell Ig-like Receptors (KIR) to their Class I MHC ligands was shown previously to be characterized by extremely rapid association and dissociation rate constants. During experiments to investigate the biochemistry of receptor-ligand binding in more detail, the kinetic parameters of the interaction were observed to alter dramatically in the presence of Zn2+ but not other divalent cations, The basis of this phenomenon is Zn2+-induced multimerization of the KIR molecules as demonstrated by BIAcore, analytical ultracentrifugation, and chemical cross-linking experiments. Zn2+-dependent multimerization of KIR may be critical for formation of the clusters of KIR and HLA-C molecules, the "natural killer (NK) cell immune synapse," observed at the site of contact between the NK cell and target cell.