Variations in functional immunocompetence of individual tumor-draining lymph nodes in humans.
Variations in functional immunocompetence of individual tumor-draining lymph nodes in humans.
复制标题
人类个体肿瘤引流淋巴结功能性免疫能力的变化。
作者：
D. Hoon;E. Korn;A. Cochran
在肿瘤引流淋巴结的组织学和免疫组织学研究中，我们观察到靠近肿瘤的淋巴结相对于远离肿瘤的淋巴结显示出降低的副皮质活性。为了评估这种减少是否是由淋巴结功能活动的改变引起的，我们检查了单个定向的肿瘤引流淋巴结的免疫活性。我们评估了淋巴结淋巴细胞的未受刺激的活性及其对植物血凝素、白细胞介素2和单向同种异体抗原刺激（混合淋巴细胞反应）的反应。恶性黑色素瘤或乳腺癌患者的区域淋巴结被分类为相对于肿瘤的近端、中间或远端。对有无肿瘤转移的淋巴结组进行了研究。同一个体不同淋巴结的未刺激和刺激淋巴细胞的[3 H]胸苷摄取存在显著差异。靠近肿瘤的淋巴结的反应比那些位于较远的，后者中的一些显示出相对的免疫刺激。因此，肿瘤引流淋巴结组显示了单个淋巴结反应强度的非随机变化。存在低和高淋巴结反应性区域，其与每个淋巴结相对于肿瘤的位置相关。肿瘤源性免疫抑制产物和/或免疫调节下调淋巴结功能活性，产生可能允许肿瘤细胞存活和转移建立的条件。作者认为，免疫抑制邻近淋巴结可能有助于早期转移。
In studying the histology and immunohistology of tumor-draining lymph nodes, we observed that nodes close to tumor showed reduced paracortical activity relative to those further away. To assess whether this reduction was paralleled by alterations in the functional activity of nodes, we examined the immunocompetence of individually oriented tumor-draining lymph nodes. We assessed the unstimulated activity of lymph node lymphocytes and their responses to phytohemagglutinin, interleukin 2, and one-way alloantigenic stimulation (mixed lymphocyte reaction) in vitro. Regional nodes from patients with malignant melanoma or breast cancer were classified as proximal, intermediate, or distal relative to tumor. Node groups with and without tumor metastases in them were studied. Significant variations in [3H]thymidine uptake were noted with the unstimulated and stimulated lymphocytes of different lymph nodes from the same individual. Nodes near to tumor were less responsive than those located further away; some of the latter showed relative immunostimulation. Tumor-draining node groups thus demonstrated a nonrandom variation in the strength of reaction of individual nodes. There are zones of low and high lymph node reactivity, related to the position of each node relative to tumor. Tumor-derived immunosuppressive products and/or immunoregulation down-regulates lymph node functional activity, creating conditions that may permit the survival of tumor cells and the establishment of metastases. It is suggested that immunosuppression of nodes nearest to tumor may facilitate the early establishment of metastases.