Proinflammatory and osteoclastogenic effects of beta-tricalciumphosphate and hydroxyapatite particles on human mononuclear cells in vitro
Proinflammatory and osteoclastogenic effects of beta-tricalciumphosphate and hydroxyapatite particles on human mononuclear cells in vitro
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DOI：
10.1016/j.biomaterials.2009.06.023
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发表时间：
2009-10-01
期刊：
影响因子：
14
通讯作者：
Amling, Michael
中科院分区：
文献类型：
--
作者：
Lange, Tobias;Schilling, Arndt F.;Amling, Michael
颗粒磨损碎屑可激活骨-种植体界面处的防御细胞和破骨细胞，可能导致骨吸收和种植体失效。已经报道了颗粒羟基磷灰石（HA）的细胞反应和炎症效应。然而，颗粒β-磷酸三钙（β-TCP）的免疫学效应尚未研究，β-TCP在这方面是否像HA一样更具生物相容性的问题仍有待确定。因此，目前的工作调查的影响，无内毒素HA和β-TCP颗粒相同的大小（d（50）= 1 μ m）和剂量（SAR 10：1）对人外周血单核细胞在体外。促炎细胞因子（TNF α. IL-1 β、IL-8）和与破骨细胞和树突状细胞分化相关的细胞因子（OPG、RANKL、M-CSF、GM-CSF）。在孵育6和18小时后，HA和β-TCP对TNF-α、IL-1 β和IL-8的诱导作用非常相似。未检测到颗粒对M-CSF和OPG产生的影响。然而，与HA形成鲜明对比的是，β-TCP引起较少的GM-CSF诱导，而不是任何RANKL，这两者分别促进树突状细胞和破骨细胞生成。因此，这些体外数据表明，β-TCP的磨屑对HA已知的破骨细胞诱导的有害影响的风险较小。(C)2009爱思唯尔有限公司版权所有。
Particulate wear debris can activate defence cells and osteoclasts at the bone-implant inter-face possibly leading to bone resorption and implant failure. Cellular responses and inflammatory effects have been reported for particulate hydroxyapatite (HA). However, the immunological effects of particulate beta-tricalciumphosphate (beta-TCP) have not been studied and the question of whether beta-TCP is more biocompatible in this regard as is HA remains to be determined. Therefore the present work investigates effects of endotoxin-free HA and beta-TCP particles of the same size (d(50) = 1 mu m) and dose (SAR 10:1) on human peripheral blood mononuclear cells in vitro. The production of proinflammatory cytokines (TNFalpha. IL-1beta, IL-8) and cytokines connected to osteoclast and dendritic cell differentiation (OPG, RANKL, M-CSF, GM-CSF) was determined by ELISA. After 6 and 18 h of incubation HA and beta-TCP caused a quite similar induction of TNF-alpha, IL-1beta and IL-8. Effects of particles on the production of M-CSF and OPG were not detectable. However, in sharp contrast to HA, beta-TCP caused less induction of GM-CSF and not any of RANKL, both known for promoting dendritic cells and osteoclastogenesis respectively. Therefore these in vitro data suggest that wear debris of beta-TCP poses lesser risk of the detrimental effects of osteoclast induction known from HA. (C) 2009 Elsevier Ltd. All rights reserved.