Non-psychoactive cannabinoids identification by linear retention index approach applied to a hand-portable capillary liquid chromatography platform
Non-psychoactive cannabinoids identification by linear retention index approach applied to a hand-portable capillary liquid chromatography platform
复制标题
应用于手持式毛细管液相色谱平台的线性保留指数法鉴定非精神活性大麻素
DOI：
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发表时间：
2022
影响因子：
4.3
通讯作者：
L. Mondello
中科院分区：
文献类型：
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作者：
Roberta La Tella;Francesca Rigano;P. Guarnaccia;P. Dugo;L. Mondello
本研究的目的是应用线性保留指数（LRI）系统的非精神活性大麻素的鉴定使用便携式LC仪器。小型化，即使用非常少量的移动的相，使得能够开发用于原位分析的紧凑型移动的系统，也是根据绿色和节省成本的方法。特别是，新的毛细管液相色谱法（cap-LC）与紫外检测耦合的分析大麻提取物的发展。探索了两种设置以实现24种大麻素的有效分离：单柱设置，其利用低于2 μm的填料来提高色谱分辨率，以及基于两种不同固定相的串联连接的双柱设置，每个固定相与UV检测器耦合。后者允许确定两个LRI值为每种分析物，从而提高了识别能力。此外，由于在基于LED的紫外线检测器上使用了两种不同的波长，在每种色谱痕迹上测量到的紫外线的比率代表了第三种鉴别标准，从而满足了分析缉获毒品科学工作组关于所使用的分析技术的类别和明确鉴别毒品所需的最低数量参数的建议。所获得的结果可用于开发一种新的分析方法，用于快速和自动化的现场法医调查和大麻育种计划，同时最大限度地减少样品和溶剂的消耗。
The aim of the present research was the application of the linear retention index (LRI) system for the identification of non-psychoactive cannabinoids using a portable LC instrument. The miniaturization, viz. the use of very low quantities of mobile phase, enabled the development of a compact mobile system to be used for in situ analysis, also according to a green and cost-saving approach. In particular, new capillary LC (cap-LC) methods coupled with UV detection were developed for the analysis of extracts of Cannabis sativa L. Two setups were explored to achieve the efficient separation of twenty-four cannabinoids: a single column setup which exploited a sub-2 µm packing to increase the chromatographic resolution, and a dual-column setup based on the serial connection of two different stationary phases, each coupled to an UV detector. The latter allowed the determination of two LRI values for each analyte, thus increasing the identification power. Moreover, since two different wavelengths were used on the LED-based UV detectors, the ratio of the absorbances measured on each chromatographic trace represented a third identification criterion, thus fulfilling the recommendations of the Scientific Working Group for The Analysis of Seized Drugs (SWDRUG) about the categories of analytical techniques to be used and the minimum number of parameters required for the unambiguous identification of drugs. The obtained results could be used for the development of a novel analytical method for fast and automatic in situ forensic investigations and hemp breeding programs, also minimizing the consumption of both sample and solvent.
通过高效液相色谱-串联质谱法测定吸入大麻后小鼠脑中大麻环烯、大麻二酚和α-四氢大麻酚及其代谢物的处置。
影响因子：
2.5
作者：
Poklis,JustinL;Thompson,CandaceC;Long,KellyA;Lichtman,AronH;Poklis,Alphonse
通讯作者：
Poklis,Alphonse