Quantitative LSPR Imaging for Biosensing with Single Nanostructure Resolution
Quantitative LSPR Imaging for Biosensing with Single Nanostructure Resolution
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DOI：
10.1016/j.bpj.2012.11.3821
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发表时间：
2013-01-08
影响因子：
3.4
通讯作者：
Byers, Jeff M.
中科院分区：
文献类型：
--
作者：
Raphael, Marc P.;Christodoulides, Joseph A.;Byers, Jeff M.
局部表面等离子体共振（LSPR）成像具有绘制分析物浓度的复杂时空变化的潜力，例如由活细胞的蛋白质分泌物产生的那些。实现这些应用的一个根本障碍是校准用于定量分析的纳米级传感器的挑战。在这里，我们介绍了一种新的，据我们所知，LSPR成像和分析技术，使数百个单独的金纳米结构的平行校准。校准使我们能够以纳摩尔灵敏度和225 ms的时间分辨率绘制单个纳米结构处的表面结合受体的部分占用率。作为该技术对分子和细胞生物学的适用性的证明，校准的阵列用于抗c-从培养的9 E10杂交瘤细胞系收获myc抗体，而不需要进一步纯化或处理。
Localized surface plasmon resonance (LSPR) imaging has the potential to map complex spatio-temporal variations in analyte concentration, such as those produced by protein secretions from live cells. A fundamental roadblock to the realization of such applications is the challenge of calibrating a nanoscale sensor for quantitative analysis. Here, we introduce a new, to our knowledge, LSPR imaging and analysis technique that enables the calibration of hundreds of individual gold nanostructures in parallel. The calibration allowed us to map the fractional occupancy of surface-bound receptors at individual nanostructures with nanomolar sensitivity and a temporal resolution of 225 ms. As a demonstration of the technique's applicability to molecular and cell biology, the calibrated array was used for the quantitative LSPR imaging of anti-c-myc antibodies harvested from a cultured 9E10 hybridoma cell line without the need for further purification or processing.