Optical microangiography provides an ability to monitor responses of cerebral microcirculation to hypoxia and hyperoxia in mice
Optical microangiography provides an ability to monitor responses of cerebral microcirculation to hypoxia and hyperoxia in mice
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DOI：
10.1117/1.3625238
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发表时间：
2011-09-01
影响因子：
3.5
通讯作者：
Wang, Ruikang K.
中科院分区：
文献类型：
--
作者：
Jia, Yali;Li, Peng;Wang, Ruikang K.
微循环的体内成像可以提高我们对缺氧和高氧等各种生理挑战下脑微血流动力学的基本了解。然而，现有的技术往往涉及使用侵入性程序或外源性造影剂，这将不可避免地干扰微循环的内在生理反应正在调查。我们报告了无标记监测吸氧引起的微循环反应的光学微血管造影（OMAG）。我们首次证明，OMAG能够显示急性缺氧和高氧对微血流动力学活动的影响，包括啮齿动物体内毛细血管和非毛细血管内微血管密度和流量调节的被动和主动调节。OMAG的功能成像的完整的微循环的能力，为研究脑疾病的未来应用前景。(C)2011年，美国光电仪器工程师学会（SPIE）。[DOI电话：10.1117/1.3625238]
In vivo imaging of microcirculation can improve our fundamental understanding of cerebral microhemodynamics under various physiological challenges, such as hypoxia and hyperoxia. However, existing techniques often involve the use of invasive procedures or exogenous contrast agents, which would inevitably perturb the intrinsic physiologic responses of microcirculation being investigated. We report ultrahigh sensitive optical microangiography (OMAG) for label-free monitoring of microcirculation responses challenged by oxygen inhalation. For the first time, we demonstrate that OMAG is capable of showing the impact of acute hypoxia and hyperoxia on microhemodynamic activities, including the passive and active modulation of microvascular density and flux regulation, within capillary and noncapillary vessels in rodents in vivo. The ability of OMAG to functionally image the intact microcirculation promises future applications for studying cerebral diseases. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3625238]