DHR3, an ecdysone-inducible early-late gene encoding a Drosophila nuclear receptor, is required for embryogenesis
DHR3, an ecdysone-inducible early-late gene encoding a Drosophila nuclear receptor, is required for embryogenesis
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DOI：
10.1073/pnas.94.22.12024
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发表时间：
1997-10-28
影响因子：
11.1
通讯作者：
Bender, M
中科院分区：
文献类型：
--
作者：
Carney, GE;Wade, AA;Bender, M
果蝇对类固醇激素脱皮激素的反应受遗传调控等级控制，包括核受体蛋白家族的8个成员，DHR3基因，位于46F早-晚脱皮激素诱导染色体泡；编码一种孤儿核受体，该受体最近被证明在蜕皮激素诱导的变态遗传等级中发挥积极和消极的调节作用。我们使用反向遗传学方法从第二条染色体46F区的致死性突变库中鉴定出11个DHR3突变，两个DHR3突变导致保守的DIVA结合域内的氨基酸替换，DHR3突变分析表明，DHR3的功能是完成胚胎发生所必需的，所有检测的DHR3等位基因都导致胚胎神经系统缺陷。
Response to the steroid hormone ecdysone in Drosophila is controlled by genetic regulatory hierarchies that include eight members of the nuclear receptor protein family, The DHR3 gene, located within the 46F early-late ecdysone-inducible chromosome puff; encodes an orphan nuclear receptor that recently has been shown to exert both positive and negative regulatory effects in the ecdysone-induced genetic hierarchies at metamorphosis. We used a reverse genetics approach to identify 11 DHR3 mutants from a pool of lethal mutations in the 46F region on the second chromosome, Two DHR3 mutations result in amino acid substitutions within the conserved DIVA binding domain, Analysis of DHR3 mutants reveals that DHR3 function is required to complete embryogenesis, All DHR3 alleles examined result in nervous system defects in the embryo.