Placental protein 13 (PP-13): Effects on cultured trophoblasts, and its detection in human body fluids in normal and pathological pregnancies
Placental protein 13 (PP-13): Effects on cultured trophoblasts, and its detection in human body fluids in normal and pathological pregnancies
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DOI：
10.1016/j.placenta.2003.12.009
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发表时间：
2004-08-01
期刊：
影响因子：
3.8
通讯作者：
Gonen, R
中科院分区：
文献类型：
--
作者：
Burger, O;Pick, E;Gonen, R
胎盘组织蛋白13(PP-13)是目前已知的56种胎盘蛋白之一，它是从分娩时从孕妇的胎盘中提纯出来的，并用来激发抗体。将纯化的PP-13裂解成多肽，测序后进行全长cDNA测序，并在大肠杆菌中表达。数据库中的序列分析表明与半乳糖凝集素家族同源。在开发的各种抗体制剂中，一对与重组PP-13(PP-13-R)偶联的单抗用于固相ELISA法检测孕妇血清中的PP-13。非孕妇女血清和男性血清中PP-13的动态范围为25~500 pg/m L，无背景，适用于妊娠1、2、3周的PP-13的检测。PP-13水平在孕期缓慢升高。在妊娠早期，胎儿生长受限(IUGR)、子痫前期(PE)，尤其是早孕(孕34周)PP-13水平低于正常。PP-13作用于培养的滋养层细胞引起钙离子携带的去极化，亚油酸和花生四烯酸从滋养层膜释放，前列环素和血栓素升高。当使用来自IUGR、PE或PTD患者胎盘的PP-13时，这些影响可以忽略不计。考虑到PP-13可能用于早期血清筛查以评估发生胎盘功能不全的风险，并通过分析培养的滋养层细胞对各种病理进行差异分析，因此对结果进行了讨论。(C)2004爱思唯尔有限公司。保留所有权利。
Placental tissue protein 13 (PP-13), one of the 56 known placental proteins identified till today, was purified from placentas obtained from women at delivery, and used to evoke antibodies against it. The purified PP-13 was lysed to peptides, which were sequenced, leading to the full-length cDNA sequencing and its expression in Escherichia coli. Sequence analysis in databases showed homology to the galectin family.Of the various antibody preparations developed, a pair of monoclonal antibodies (MAbs) coupled to the recombinant PP-13 (PP-13-R) was used for the immunodetection of PP-13 in pregnant women's serum with the solid-phase ELISA format. With a dynamic range of 25-500 pg/mL with no background in non-pregnant women's serum and men's serum, the ELISA test was suitable for the detection of PP-13 in the 1st, 2nd, and 3rd trimesters. PP-13 levels slowly increase during pregnancy. In the 1st trimester, lower than normal PP-13 levels were found in fetal growth restriction (IUGR), preeclampsia (PE), and particularly in early PE (< 34 weeks of gestation). In the 2nd and 3rd trimesters, higher than normal concentrations were found in PE, IUGR and in preterm delivery (PTD).Application of PP-13 to cultured trophoblasts elicited depolarization carried by calcium ions, followed by liberation of linoleic and arachidonic acids from the trophoblast membrane, and a subsequent elevation of prostacyclin and thromboxane. These effects were negligible when PP-13 derived from the placentas of patients with IUGR, PE or PTD was used. The results are discussed in view of the potential utilization of PP-13 for early serum screening to assess the risk to develop placental insufficiency, coupled to a differential analysis of the various pathologies by analyzing cultured trophoblasts. (C) 2004 Elsevier Ltd. All rights reserved.