REPEATED HEXANUCLEOTIDE C-C-C-C-A-A IS PRESENT NEAR FREE ENDS OF MACRONUCLEAR DNA OF TETRAHYMENA
REPEATED HEXANUCLEOTIDE C-C-C-C-A-A IS PRESENT NEAR FREE ENDS OF MACRONUCLEAR DNA OF TETRAHYMENA
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DOI：
10.1073/pnas.78.12.7436
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
YAO, CH
中科院分区：
文献类型：
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作者：
YAO, MC;YAO, CH
在T.嗜热菌此外，在体细胞大核的重复序列的这些集群的组织是不同的，在germinal微核。外切核酸酶Bal 31用于显示重复位于大核中DNA的游离端附近。当用Bal 31消化全细胞DNA或大核DNA以从游离末端去除约600个碱基对时，通过杂交判断，去除了80%的C4A2重复。没有特定的簇对外切核酸酶消化具有抗性，这表明基本上所有的C4A2重复序列都位于DNA的游离端附近。另一方面，微核中的C4A2重复序列未被Bal 31消化。
The tandemly repeated hexanucleotide C-C-C-C-A-A has previously been found near the termini of extrachromosomal gene coding for rRNA and in many other locations of the genome of T. thermophila. Moreover, the organization of these clusters of repeats in the somatic macronucleus is different from that in the germinal micronucleus. Exonuclease Bal 31 was used to show that the repeats are located near free ends of DNA in the macronucleus. When whole cell DNA or macronuclear DNA was digested with Bal 31 to remove approximately 600 base pairs from free ends, 80% of the C4A2 repeats were removed, as judged by hybridization. No particular cluster was resistant to exonuclease digestion, suggesting that essentially all the C4A2 repeats are located near free ends of DNA. The C4A2 repeats in the micronucleus, on the other hand, were not digested by Bal 31.