The 5' end of U3 snRNA can be crosslinked in vivo to the external transcribed spacer of rat ribosomal RNA precursors.
The 5' end of U3 snRNA can be crosslinked in vivo to the external transcribed spacer of rat ribosomal RNA precursors.
复制标题
U3 snRNA 的 5 末端可以在体内与大鼠核糖体 RNA 前体的外部转录间隔区交联。
DOI：
10.1016/0022-2836(89)90126-5
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发表时间：
1989
影响因子：
5.6
通讯作者：
Weiner,AM
中科院分区：
文献类型：
--
作者：
Stroke,IL;Weiner,AM
在4′-氨甲基-4，5 ′，8-三甲基芘烯（AMT peptien）存在下，经长波紫外线照射，U3 RNA与核仁28 S ~ 35 S RNA形成氢键，并与>28 S的RNA共价交联。在这里，我们使用一种新的夹心印迹技术来鉴定这些大的核仁RNA种类作为rRNA前体，并绘制体内交联位点。U3 RNA的5′端附近的一个或多个残基与大鼠rRNA外转录间隔区（ETS 1）的380个核苷酸区域之间发生交联。我们对大鼠ETS的这一区域进行了测序，发现它包括一个RNA加工位点，该位点类似于先前定位在小鼠和人18 S rRNA 5′端上游约3.5 kb处的位点。
From previous work it was known that U3 RNA is hydrogen bonded to nucleolar 28 S to 35 S RNA and can be covalently crosslinked to RNA of >28 S by irradiationin vivowith long-wave ultraviolet light in the presence of 4′-aminomethyl-4,5′,8-trimethylpsoralen (AMT psoralen). Here we use a novel sandwich blot technique to identify these large nucleolar RNA species as rRNA precursors and to map the site(s) of crosslinkingin vivo. The crosslink occurs between one or more residues near the 5′ end of U3 RNA and a 380 nucleotide region of the rat rRNA external transcribed spacer (ETS1). We have sequenced this region of the rat ETS and we show that it includes an RNA-processing site analogous to those previously mapped to approximately 3.5 kb upstream from the 5′ end of mouse and human 18 S rRNAs.