Bacterial biota in the human distal esophagus
Bacterial biota in the human distal esophagus
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DOI：
10.1073/pnas.0306398101
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发表时间：
2004-03-23
影响因子：
11.1
通讯作者：
Blaser, MJ
中科院分区：
文献类型：
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作者：
Pei, ZH;Bini, EJ;Blaser, MJ
与消化系统的其他腔器官一样，食道为细菌定植提供了潜在的环境，但人们对细菌生物群的存在及其性质知之甚少。应用广谱16S rDNA聚合酶链式反应技术，对4例成人正常食道组织进行了检测。所克隆的900个PCR产物代表了833个独特的序列，隶属于41个属，或95个种水平的操作分类单位(SLOTU)；59个SLOTU与培养定义的细菌物种同源，34个与16S rDNA克隆同源，2个与任何已知细菌16S rDNA同源。代表了六个门的成员，即菲尔米库特、类杆菌、放线杆菌、变形杆菌、梭杆菌和TM7。绝大多数克隆属于四人共有的41个属中的13个(783/900，87%)或14个SLOTU(574/900，%)。以链球菌属(39%)、普氏杆菌属(17%)和韦洛氏菌属(14%)为主。目前的研究确定了这个细菌生态系统中大约56-79%的SLOTU。大多数食道生物群的SLOTU与上游口腔生物群的居民相似或相同，但主要的区别是绝大多数(82%)的食道细菌是已知和可培养的。这些发现为正常的远端食道中存在复杂但保守的细菌种群提供了证据。
The esophagus, like other luminal organs of the digestive system, provides a potential environment for bacterial colonization, but little is known about the presence of a bacterial biota or its nature. By using broad-range 16S rDNA PCR, biopsies were examined from the normal esophagus of four human adults. The 900 PCR products cloned represented 833 unique sequences belonging to 41 genera, or 95 species-level operational taxonomic units (SLOTU); 59 SLOTU were homologous with culture-defined bacterial species, 34 with 16S rDNA clones, and two were not homologous with any known bacterial 16S rDNA. Members of six phyla, Firmicutes, Bacteroides, Actinobacteria, Proteobacteria, Fusobacteria, and TM7, were represented. A large majority of clones belong to 13 of the 41 genera (783/900, 87%), or 14 SLOTU (574/900, 64%) that were shared by all four persons. Streptococcus (39%), Prevotella (17%), and Veilonella (14%) were most prevalent. The present study identified approximate to56-79% of SLOTU in this bacterial ecosystem. Most SLOTU of esophageal biota are similar or identical to residents of the upstream oral biota, but the major distinction is that a large majority (82%) of the esophageal bacteria are known and cultivable. These findings provide evidence for a complex but conserved bacterial population in the normal distal esophagus.