Biomimetic collagen scaffolds with anisotropic pore architecture
Biomimetic collagen scaffolds with anisotropic pore architecture
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DOI：
10.1016/j.actbio.2011.09.033
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发表时间：
2012-02-01
影响因子：
9.7
通讯作者：
Cameron, R. E.
中科院分区：
文献类型：
--
作者：
Davidenko, N.;Gibb, T.;Cameron, R. E.
海绵样基质具有类似于特定组织的实际细胞外基质的特定三维结构设计，显示出再生和修复广泛的受损各向异性组织的显著潜力。在这项工作中，探索了使用冷冻干燥技术的胶原蛋白支架的结构的操纵作为定制支架的内部结构的固有生物相容性的方式。该研究集中在温度梯度的影响，施加在胶原悬浮液的结晶阶段，在所产生的支架的微观结构的各向异性程度。采用成型技术来实现在冷冻过程中的传热速率的差异。为此，开发了具有不同配置的各种模具，以便在该过程中在样品上产生单轴和多方向的温度梯度。扫描电子显微镜分析不同的横截面（纵向和水平）的支架显示，高度对齐的矩阵与轴向定向的孔结构，获得单一的单向温度梯度诱导。通过引入多个温度梯度来改变冷冻条件，允许产生具有复杂孔取向以及孔尺寸和排列的各向异性的胶原蛋白支架。皇冠版权所有（C）2011由爱思唯尔有限公司代表Acta Materialia Inc.出版。All rights reserved.
Sponge-like matrices with a specific three-dimensional structural design resembling the actual extracellular matrix of a particular tissue show significant potential for the regeneration and repair of a broad range of damaged anisotropic tissues. The manipulation of the structure of collagen scaffolds using a freeze-drying technique was explored in this work as an intrinsically biocompatible way of tailoring the inner architecture of the scaffold. The research focused on the influence of temperature gradients, imposed during the phase of crystallisation of collagen suspensions, upon the degree of anisotropy in the microstructures of the scaffolds produced. Moulding technology was employed to achieve differences in heat transfer rates during the freezing processes. For this purpose various moulds with different configurations were developed with a view to producing uniaxial and multi-directional temperature gradients across the sample during this process. Scanning electron microscopy analysis of different cross-sections (longitudinal and horizontal) of scaffolds revealed that highly aligned matrices with axially directed pore architectures were obtained where single unidirectional temperature gradients were induced. Altering the freezing conditions by the introduction of multiple temperature gradients allowed collagen scaffolds to be produced with complex pore orientations, and anisotropy in pore size and alignment. Crown Copyright (C) 2011 Published by Elsevier Ltd. on behalf of Acta Materialia Inc. All rights reserved.