Contractile proteins in pericytes. I. Immunoperoxidase localization of tropomyosin
Contractile proteins in pericytes. I. Immunoperoxidase localization of tropomyosin
复制标题
周细胞中的收缩蛋白。
作者：
N. Joyce;M. F. Haire;G. Palade
在这些研究中，我们比较了四种大鼠微血管床（心脏、膈肌、胰腺和肠粘膜）中毛细血管和毛细血管后小静脉周细胞、内皮细胞和血管平滑肌细胞中原肌球蛋白的相对含量和亚型。通过原位免疫过氧化物酶定位获得的结果表明，（a）原肌球蛋白以相对高的浓度存在于毛细血管和毛细血管后小静脉周细胞中； (b) 周细胞的原肌球蛋白含量似乎略低于血管平滑肌细胞中的含量，但高于内皮细胞和其他血管相关细胞中的含量； (c) 与内皮细胞和其他非肌肉细胞不同，周细胞含有可检测水平的与平滑肌亚型免疫相关的原肌球蛋白。这些结果和我们之前关于周细胞中环 GMP 依赖性蛋白激酶的存在的发现（Joyce，N.，P. DeCamilli，和 J. Boyles，1984，Microvasc. Res. 28：206-219）证明这些细胞含有大量的至少两种对于收缩调节重要的蛋白质。综上所述，证据表明周细胞是与血管平滑肌细胞相关的收缩元件，可能与后者一样参与微脉管系统血流的调节。
In these studies we have compared the relative amounts and isoforms of tropomyosin in capillary and postcapillary venule pericytes, endothelial cells, and vascular smooth muscle cells in four rat microvascular beds: heart, diaphragm, pancreas, and the intestinal mucosa. The results, obtained by in situ immunoperoxidase localization, indicate that (a) tropomyosin is present in capillary and postcapillary venule pericytes in relatively high concentration; (b) the tropomyosin content of pericytes appears to be somewhat lower than in vascular smooth muscle cells but higher than in endothelia and other vessel- associated cells; and (c) pericytes, unlike endothelia and other nonmuscle cells, contain detectable levels of tropomyosin immunologically related to the smooth muscle isoform. These results and our previous findings concerning the presence of a cyclic GMP-dependent protein kinase (Joyce, N., P. DeCamilli, and J. Boyles, 1984, Microvasc. Res. 28:206-219) in pericytes demonstrate that these cells contain significant amounts of at least two proteins important for contraction regulation. Taken together, the evidence suggests that pericytes are contractile elements related to vascular smooth muscle cells, possibly involved, as are the latter, in the regulation of blood flow through the microvasculature.
大鼠红色肌肉中周细胞的扫描电子显微镜。
影响因子：
3.1
作者：
Mazanet,R;Franzini-Armstrong,C
通讯作者：
Franzini-Armstrong,C
视网膜周细胞和内皮细胞中的肌动蛋白丝。
影响因子：
4.4
作者：
Wallow,IH;Burnside,B
通讯作者：
Burnside,B