Analysis of Plasmodium falciparum infections in a village community in Northern Nigeria:: determination of msp2 genotypes and parasite-specific IgG responses
Analysis of Plasmodium falciparum infections in a village community in Northern Nigeria:: determination of msp2 genotypes and parasite-specific IgG responses
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DOI：
10.1016/s0001-706x(99)00044-3
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发表时间：
2000-01-05
期刊：
影响因子：
2.7
通讯作者：
Felger, I
中科院分区：
文献类型：
--
作者：
Engelbrecht, F;Tögel, E;Felger, I
在雨季结束时，传播率很高，在尼日利亚北部的一个村庄对恶性疟原虫的遗传多样性和感染多重性进行了研究。我们通过聚合酶链式反应 (PCR) 扩增裂殖子表面蛋白 MSP2 的基因，然后基于限制性片段长度多态性 (RFLP) 进行基因分型，分析了 104 名 5-70 岁个体的血液样本。 PCR检测显示，94.2%的样本呈寄生虫阳性，其中80%以上存在多重感染。恶性疟原虫感染中存在的寄生虫克隆的平均数量的年龄分布表现出最初的增加，然后在8-10岁的儿童中达到峰值，然后随着年龄的增长而显着下降。这些 8-10 岁儿童的平均多重性为每个携带者 5.4 个克隆。将尼日利亚个体的峰值多样性和寄生虫多样性与非洲和巴布亚新几内亚其他研究地点的发现进行比较。环子孢子蛋白 (CSP) 的 IgG 抗体（疟疾暴露指标）在所有年龄组中的流行率超过 85%，表明村民对恶性疟原虫的暴露程度很高。 ELISA OD 值与年龄呈正相关。 msp2 PCR 测定的抗 CSP IgG 水平与恶性疟原虫感染复数之间没有相关性。这些结果表明，在高流行地区，感染的复数性与接触恶性疟原虫并不直接相关。 (C) 2000 Elsevier Science B.V. 保留所有权利。
The genetic diversity of P. falciparum and multiplicity of infection has been studied in a village in Northern Nigeria at the end of the rainy season, when transmission is high. We analysed blood samples from 104 individuals aged 5-70 years by polymerase chain reaction (PCR) amplifying the gene for the merozoite surface protein MSP2 followed by genotyping based on restriction fragment length polymorphism (RFLP). 94.2% of all samples were parasite positive by PCR and over 80% of those had multiple infections. The age distribution of the average number of parasite clones present in P. falciparum infections showed an initial increase, then reached a peak multiplicity in children 8-10 years of age, and afterwards decreased significantly with age. Mean multiplicity in those 8-10-year-old children was 5.4 clones per carrier. Peak multiplicity and parasite diversity in Nigerian individuals is compared to findings from other study sites in Africa and PNG. The prevalence of IgG antibodies against the circumsporozoite protein (CSP), an indicator for malaria exposure, was over 85% in all age groups showing a high exposure of villagers to P. falciparum. OD values in ELISA were positively correlated with age. There was no correlation between the level of IgG against CSP and the multiplicity of P. falciparum infections determined by PCR of msp2. These results imply that in highly endemic areas multiplicity of infection is not directly correlated with exposure to P. falciparum. (C) 2000 Elsevier Science B.V. All rights reserved.