Genome-wide survey and analysis of the TIFY gene family and its potential role in anthocyanin synthesis in Chinese sand pear (Pyrus pyrifolia)
Genome-wide survey and analysis of the TIFY gene family and its potential role in anthocyanin synthesis in Chinese sand pear (Pyrus pyrifolia)
复制标题
梨叶TIFY基因家族全基因组调查分析及其在花色苷合成中的潜在作用
DOI：
10.1007/s11295-018-1240-6
复制
发表时间：
2018-04-01
影响因子：
2.4
通讯作者：
Teng, Yuanwen
中科院分区：
文献类型：
--
作者：
Ma, Yunjing;Shu, Shashan;Teng, Yuanwen
TIFY家族是植物中特有的一个重要基因家族，参与多种生物学过程。茉莉酸（JA）促进果实中花色素苷的积累。为探讨PpTIFY基因在砂梨花色素苷合成中的作用，本研究首先从砂梨基因组中克隆了21个PpTIFY基因，并进一步鉴定了11个PpJAZ基因。PpTIFY基因序列相似性较低，表明高等植物中的PpTIFY基因在陆生植物进化过程中分化较早，经历了较大的突变。PpTIFY基因在梨各器官组织中均有表达。PpTIFY基因的时空表达模式表明，PpTIFY基因与花色素苷积累和茉莉酸信号传导有关。PpTIFY基因在叶片中的表达量最高，而在果实成熟过程中，表达量急剧下降。此外，茉莉酸和光照处理后，与花青苷的积累，在中国砂梨红果果皮中诱导PpTIFY。
The TIFY family is an important gene family which can be found only in plant and involved in different biologic processes. Jasmonic acid (JA) promotes anthocyanin accumulation in fruit. To explore the role of PpTIFY genes in anthocyanin biosynthesis of Chinese sand pear (Pyrus pyrifolia Nakai), we first cloned 21 PpTIFY genes from the pear genome and further identified 11 PpJAZ genes. The sequence similarity among the PpTIFY genes was relatively low, which indicated that PpTIFY genes in higher plants differentiated early during land plant evolution and have experienced considerable mutation. Transcripts of PpTIFY genes were detected in all organs and tissues of pear analyzed. The spatial and temporal expression patterns indicated that PpTIFY genes were associated with anthocyanin accumulation and JA signaling. The expression levels of PpTIFY genes were highest in leaves, whereas during fruit maturation, the expression level dramatically decreased. Furthermore, PpTIFY was induced after JA and light treatment in conjunction with anthocyanin accumulation in the peel of red fruit of Chinese sand pear.