The gene fl(2)d is needed for the sex-specific splicing of transformer pre-mRNA but not for double-sex pre-mRNA in Drosophila melanogaster
The gene fl(2)d is needed for the sex-specific splicing of transformer pre-mRNA but not for double-sex pre-mRNA in Drosophila melanogaster
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DOI：
10.1007/s004380050292
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发表时间：
1996-11-27
期刊：
影响因子：
--
通讯作者：
Sanchez, L
中科院分区：
文献类型：
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作者：
Granadino, B;Penalva, LOF;Sanchez, L
在黑腹果蝇中，性别决定基因在整个发育过程中的调控是通过其产物的性别特异性剪接发生的。第一个基因是性致死基因（Sex-lethal，Sri）。Sxl的下游靶标是基因Transformer（tra）：Sxl蛋白控制Tra前mRNA的雌性特异性剪接。基因aa的下游靶基因是基因双性（dsx）：雌性的Tra蛋白，控制Dsx前mRNA的雌性特异性剪接。我们已经确定了一个基因，female-lethal-2-d fl（2）d，其功能是女性特异性剪接Sxl前mRNA所必需的。在这份报告中，我们分析基因fl（2）d是否也需要性别特异性剪接的Tra和Dsx前mRNA。我们发现Sri蛋白不足以进行Tra前mRNA的雌性特异性剪接，fl（2）d功能也是必需的。然而，该基因对于Dsx前mRNA的雌性特异性剪接不是必需的。
In Drosophila melanogaster, regulation of the sex determination genes throughout development occurs by sex-specific splicing of their products. The first gene is Sex-lethal (Sri). The downstream target of Sxl is the gene transformer (tra): the Sxl protein controls the female-specific splicing of the Tra pre-mRNA. The downstream target of the gene a a is the gene double-sex (dsx): the Tra protein of females, controls the female-specific splicing of the Dsx pre-mRNA. We have identified a gene, female-lethal-2-d fl (2) d, whose function is required for the female-specific splicing of Sxl pre-mRNA. In this report we analyze whether the gene fl (2) d is also required for the sex-specific splicing of both Tra and Dsx pre-mRNAs. We found that the Sri protein is not sufficient for the female-specific splicing of Tra pre-mRNA, the fl (2) d function also being necessary. This gene; however, is not required for the female-specific splicing of Dsx pre-mRNA.