PH-sensing nano-crystals of carbonate apatite: Effects on intracellular delivery and release of DNA for efficient expression into mammalian cells
PH-sensing nano-crystals of carbonate apatite: Effects on intracellular delivery and release of DNA for efficient expression into mammalian cells
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DOI：
10.1016/j.gene.2006.02.028
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发表时间：
2006-07-05
期刊：
影响因子：
3.5
通讯作者：
Akaike, T.
中科院分区：
文献类型：
--
作者：
Chowdhury, E. H.;Maruyama, A.;Akaike, T.
碳酸盐磷灰石在硬组织工程中众所周知的两种独特而迷人的特性，首次被揭示出来，用于开发最简单但最有效的非病毒基因传递装置——能够阻止高频DNA在质膜上转移所需的晶体生长，以及在内体酸化期间有效释放DNA的快速溶解速率。与广泛使用的转染剂相比，在哺乳动物细胞中具有显著的高转基因表达（5至100倍）。此外，通过加入氟化物或锶来调节碳酸盐磷灰石的晶体溶解速率，可以显著地控制转染活性，从而揭示了迄今为止被忽视的非病毒途径中的新屏障。因此，我们已经开发出一种具有重要见解的创新技术，它将成为基础研究实验室和临床环境中有前途的工具。(c) 2006 Elsevier B.V.版权所有
Two unique and fascinating properties of carbonate apatite which are well-known in hard tissue engineering, have been unveiled, for the first time, for the development of the simplest, but most efficient non-viral gene delivery device - ability of preventing the growth of crystals needed for high frequency DNA transfer across a plasma membrane and a fast dissolution rate for effective release of DNA during endosomal acidification, leading to a remarkably high transgene expression (5 to 100-fold) in mammalian cells compared to the widely used transfecting agents. Moreover, by modulating the crystal dissolution rate of carbonate apatite through incorporation of fluoride or strontium into it, transfection activity could be dramatically controlled, thus shedding light on a new barrier in the non-viral route, which was overlooked so far. Thus we have developed an innovative technology with significant insights, that would come as a promising tool for both basic research laboratories and clinical settings. (c) 2006 Elsevier B.V. All rights reserved.