Mechanistic insights into the inhibition of the CRISPR-Cas surveillance complex by anti-CRISPR protein AcrIF13.
Mechanistic insights into the inhibition of the CRISPR-Cas surveillance complex by anti-CRISPR protein AcrIF13.
复制标题
抗 CRISPR 蛋白 AcrIF13 抑制 CRISPR-Cas 监视复合物的机制见解。
DOI：
10.1016/j.jbc.2022.101636
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Feng Y
中科院分区：
文献类型：
--
作者：
Wang H;Gao T;Zhou Y;Ren J;Guo J;Zeng J;Xiao Y;Zhang Y;Feng Y
Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins provide prokaryotes with nucleic acid–based adaptive immunity against infections of mobile genetic elements, including phages. To counteract this immune process, phages have evolved various anti-CRISPR (Acr) proteins which deactivate CRISPR-Cas–based immunity. However, the mechanisms of many of these Acr-mediated inhibitions are not clear. Here, we report the crystal structure of AcrIF13 and explore its inhibition mechanism. The structure of AcrIF13 is unique and displays a negatively charged surface. Additionally, biochemical studies identified that AcrIF13 interacts with the type I-F CRISPR-Cas surveillance complex (Csy complex) to block target DNA recognition and that the Cas5f-8f tail and Cas7.6f subunit of the Csy complex are specific binding targets of AcrIF13. Further mutational studies demonstrated that several negatively charged residues of AcrIF13 and positively charged residues of Cas8f and Cas7f of the Csy complex are involved in AcrIF13–Csy binding. Together, our findings provide mechanistic insights into the inhibition mechanism of AcrIF13 and further suggest the prevalence of the function of Acr proteins as DNA mimics.
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影响因子：
16.6
作者：
Pinilla-Redondo R;Shehreen S;Marino ND;Fagerlund RD;Brown CM;Sørensen SJ;Fineran PC;Bondy-Denomy J
通讯作者：
Bondy-Denomy J
影响因子：
5.4
作者：
Tuminauskaite, Donata;Norkunaite, Danguole;Sinkunas, Tomas
通讯作者：
Sinkunas, Tomas
DOI：
10.1126/science.1159689
发表时间：
2008-08-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Brouns SJ;Jore MM;Lundgren M;Westra ER;Slijkhuis RJ;Snijders AP;Dickman MJ;Makarova KS;Koonin EV;van der Oost J
通讯作者：
van der Oost J
影响因子：
16
作者：
Niu, Yiying;Yang, Lingguang;Feng, Yue
通讯作者：
Feng, Yue
影响因子：
16.6
作者：
Hirschi, Marscha;Lu, Wang-Ting;Wiedenheft, Blake
通讯作者：
Wiedenheft, Blake