Tomato receptor-like cytosolic kinase RIPK confers broad-spectrum disease resistance without yield penalties.
Tomato receptor-like cytosolic kinase RIPK confers broad-spectrum disease resistance without yield penalties.
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DOI：
10.1093/hr/uhac207
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发表时间：
2022
影响因子：
8.7
通讯作者：
中科院分区：
文献类型：
--
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活性氧（ROS）的产生是植物多层次防御机制中的重要免疫反应，然而直接改变ROS的体内平衡来培育广谱抗病植物还没有成功。在拟南芥中，受体样胞质激酶AtRIPK调节植物免疫系统多个层次中的广谱ROS信号传导。在用免疫诱导剂处理后，AtRIPK被激活并磷酸化烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶，这导致ROS产生。在这项研究中，我们鉴定了番茄中的AtRIPK直系同源物，并使用CRISPR/Cas9技术产生了敲低突变体。Slripk突变体显示响应于代表性免疫激发子的ROS产生减少，并且对来自不同属的病原性细菌和真菌敏感，包括青枯雷尔氏菌、胡萝卜腐果胶杆菌、灰葡萄孢和尖孢镰刀菌，它们是具有半活体营养型和坏死营养型感染策略的叶和根病原体。相反，过表达SlRIPK的转基因番茄植物对这些病原体更具抗性。值得注意的是，slripk突变体和过表达Slripk的转基因植物没有表现出显著的生长迟缓或产量损失。这些结果表明，SlRIPK的过表达赋予番茄植物广谱抗病性而没有产量损失。我们的研究结果表明，通过改变植物免疫中ROS产生的调节成分来改变ROS体内平衡，可能有助于工程或育种广谱抗病作物而不会造成产量损失。
Production of reactive oxygen species (ROS) is an important immune response in plant multilayer defense mechanisms; however, direct modification of ROS homeostasis to breed plants with broad-spectrum resistance to disease has not yet been successful. In Arabidopsis, the receptor-like cytosolic kinase AtRIPK regulates broad-spectrum ROS signaling in multiple layers of the plant immune system. Upon treatment with immune elicitors, AtRIPK is activated and phosphorylates nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, which leads to ROS production. In this study, we identified an AtRIPK ortholog in tomatoes and generated knockdown mutants using CRISPR/Cas9 technology. Slripk mutants displayed reduced ROS production in response to representative immune elicitors and were susceptible to pathogenic bacteria and fungi from different genera, including Ralstonia solanacearum, Pectobacterium carotovorum, Botrytis cinerea, and Fusarium oxysporum, which are leaf and root pathogens with hemibiotrophic and necrotrophic infection strategies. In contrast, transgenic tomato plants overexpressing SlRIPK are more resistant to these pathogens. Remarkably, the slripk mutants and SlRIPK-overexpressing transgenic plants did not exhibit significant growth retardation or yield loss. These results suggest that overexpression of SlRIPK confers broad-spectrum disease resistance without a yield penalty in tomato plants. Our findings suggest that modifying ROS homeostasis by altering the regulatory components of ROS production in plant immunity could contribute to engineering or breeding broad-spectrum disease-resistant crops without yield penalty.
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影响因子：
16
作者：
Kadota, Yasuhiro;Sklenar, Jan;Zipfel, Cyril
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影响因子：
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受体样细胞质激酶 RIPK 调节植物免疫系统多层中的广谱 ROS 信号传导
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