Differentiation of preosteoblasts is affected by implant surface microtopographies
Differentiation of preosteoblasts is affected by implant surface microtopographies
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DOI：
10.1002/jbm.a.30016
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发表时间：
2004-06-01
影响因子：
4.9
通讯作者：
Stanford, C
中科院分区：
文献类型：
--
作者：
Schneider, GB;Zaharias, R;Stanford, C
骨生成涉及多能间充质细胞的募集和这些细胞向成骨细胞的渐进分化。转录因子Runx2调节成骨细胞分化和矿化表型发育所需基因的表达。本研究的目的是确定种植体表面微形貌是否影响与Runx2和骨钙素基因表达相关的前成骨细胞分化。人胚胎腭间充质细胞（HEPM细胞）培养槽或粗糙的cpTi种植盘。用落射荧光显微镜评价细胞表型。实时荧光定量PCR检测Runx2和骨钙素基因的表达。相对于组织培养塑料，HEPM细胞在粗糙和有凹槽的种植体表面上生长时矿化。实时PCR显示在粗糙和有凹槽的种植体微地形上培养的细胞中Runx2和骨钙素基因表达显著增加（p < 0.05）。这些结果表明，前成骨细胞分化的影响种植体表面的微地形在牙种植体的骨整合。(C)2004 Wiley Periodicals，Inc.
Osteogenesis involves the recruitment of multipotent mesenchymal cells and the progressive differentiation of these cells into osteoblasts. The transcription factor Runx2 regulates osteoblast differentiation and expression of genes necessary for the development of a mineralized phenotype. The purpose of this study was to determine if preosteoblast cell differentiation associated with Runx2 and osteocalcin gene expression was influenced by implant surface microtopography. Human embryonic palatal mesenchymal cells (HEPM cells) were cultured on grooved or roughened cpTi implant discs. Cell phenotypes were evaluated with epifluorescent microscopy. Real-time PCR was used for quantitative analysis of Runx2 and osteocalcin gene expression. HEPM cells mineralized when grown on rough and grooved implant surfaces relative to tissue culture plastic. Real-time PCR showed significant (p < 0.05) increases in Runx2 and osteocalcin gene expression in cells cultured on rough and grooved implant microtopographies. These results suggest that preosteoblast cell differentiation is affected by implant surface microtopographies during osseointegration of dental implants. (C) 2004 Wiley Periodicals, Inc.