Distribution characterization of microbial aerosols emitted from a wastewater treatment plant using the Orbal oxidation ditch process
Distribution characterization of microbial aerosols emitted from a wastewater treatment plant using the Orbal oxidation ditch process
复制标题
采用 Orbal 氧化沟工艺的废水处理厂排放的微生物气溶胶的分布特征
DOI：
10.1016/j.procbio.2010.12.016
复制
发表时间：
2011-04-01
影响因子：
4.4
通讯作者：
Liu, Junxin
中科院分区：
文献类型：
--
作者：
Li, Lin;Gao, Min;Liu, Junxin
采用撞击法和聚合酶链反应（PCR）法对北京市某城市污水处理厂采用Orbal氧化沟工艺的不同处理单元的生物气溶胶浓度、粒径分布和物种多样性进行了监测。VVWTP中记录的可培养细菌和真菌浓度分别为459 +/- 88至5565 +/- 571 CFU m（-3）和141 +/- 41至1590 +/- 152 CFU m（-3）。可培养细菌的浓度最高的污泥脱水房，而最高的真菌浓度被发现在细砂池（FGC）。粒径分布表明，27.6%的可培养细菌在2.1-3.3 μ m的颗粒中检测到，6.6%在0.65 - 1.1 μ m的颗粒中检测到。超过67%的真菌被检测到的颗粒大小从1.1到3.3，只有5.0%的颗粒小于1.1 μ m的大小。FGC样品中的可培养细菌群落和总空气细菌群落均表现出较高的微生物多样性。相邻处理单元产生的气载细菌显示出密切的遗传进化关系。纯培养方法和基于PCR的方法的组合可以提供额外的信息来验证生物气溶胶的存在。(c)2011爱思唯尔有限公司版权所有。
Methods based on impaction and the polymerase chain reaction (PCR) were applied to monitor the concentrations, size distributions, and species diversity of bioaerosols at different treatment units in a Beijing municipal wastewater treatment plant (WWTP) that uses an Orbal oxidation ditch process. The concentrations of culturable bacteria and fungi recorded in the VVWTP varied from 459 +/- 88 to 5565 +/- 571 CFU m(-3) and 141 +/- 41 to 1590 +/- 152 CFU m(-3), respectively. The highest concentrations of culturable bacteria were obtained in the sludge-dewatering house, whereas the highest fungi concentrations were found in the fine grit chamber (FGC). The particle size distributions showed that 27.6% of culturable bacteria were detected in particles of 2.1-3.3 mu m in size, and 6.6% were in particles between 0.65 and 1.1 mu m in size. More than 67% of the fungi were detected in particles ranging from 1.1 to 3.3 in size, with only 5.0% found in particles less than 1.1 mu m in size. Both culturable and total airborne bacterial community in FGC samples demonstrated high microbial diversities. Airborne bacteria generated from adjacent treatment units showed a close genetic evolutionary relationship. Combination of pure-culture methods and PCR-based methods can provide additional information to verify the presence of bioaerosols. (c) 2011 Elsevier Ltd. All rights reserved.