CXCL10 and CCL2 mRNA expression in monocytes is inversely correlated with the HLA-DR lower fraction of monocytes in patients with renal cell carcinoma.
CXCL10 and CCL2 mRNA expression in monocytes is inversely correlated with the HLA-DR lower fraction of monocytes in patients with renal cell carcinoma.
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DOI：
10.3892/ol.2016.4132
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发表时间：
2016-03
期刊：
影响因子：
2.9
通讯作者：
T. Motoshima;Y. Komohara;Hasita Horlad;Hirotake Tsukamoto;M. Fujita;Yoichi Saito;K. Tanoue;Yutaka Kasejima;Y. Sugiyama;Y. Kawano;Y. Nishimura;M. Takeya;M. Eto
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文献类型：
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作者：
T. Motoshima;Y. Komohara;Hasita Horlad;Hirotake Tsukamoto;M. Fujita;Yoichi Saito;K. Tanoue;Yutaka Kasejima;Y. Sugiyama;Y. Kawano;Y. Nishimura;M. Takeya;M. Eto
循环分化簇(CD)14+人类白细胞抗原(HL A)-DRlow/-单核细胞是指人类白细胞抗原(HL A)-DR低表达或阴性的单核细胞，被认为与多种恶性肿瘤患者的全身免疫抑制有关。然而，很少有研究详细研究CD14+人类白细胞抗原-DRlow/-单核细胞的基因表达谱。本研究分析了正常人和肾癌患者外周血中CD14+单核细胞免疫相关分子的表达水平。与以往的研究一致，肾癌患者CD14+单核细胞中的HLA-DRlow/-细胞百分比显著高于健康献血者。在4例手术切除原发灶的患者中，有3例术后CD14+人类白细胞抗原-DRlow/-细胞百分比显著下降。逆转录-定量聚合酶链式反应检测CD14+单核细胞环氧合酶2、转化生长因子β、白介素6R、趋化因子(C-C基序)配体2(CCL2)、趋化因子(C-X-C基序)配体10(CXCL10)、抑癌素M和血管内皮生长因子-A的基因表达水平。研究结果显示，CCL2和CXCL10的表达水平升高与CD14+HLA-DRlow/-单核细胞百分率呈负相关。这提示肾癌患者的单核细胞受到免疫抑制，肾癌患者的免疫抑制可能部分是由于循环单核细胞功能障碍所致。
Circulating cluster of differentiation (CD)14+ human leukocyte antigen (HLA)-DRlow/- monocytes, those with a lower HLA-DR expression or are negative for HLA-DR, are considered to be involved in systemic immunosuppression in patients with several malignant tumors. However, few studies have investigated in detail the gene expression profile of CD14+HLA-DRlow/- monocytes. In the present study, the mRNA expression levels of immune-associated molecules in CD14+ monocytes isolated from healthy donors and patients with renal cell carcinoma (RCC) were analyzed. Consistent with previous studies, the percentage of HLA-DRlow/- cells in CD14+ monocytes was significantly increased in patients with RCC compared with healthy donors. In 3 of the 4 patients who underwent surgical resection of the primary tumor, the percentage of CD14+HLA-DRlow/- cells was significantly decreased following surgery. The mRNA expression levels of cyclooxygenase 2, transforming growth factor β, interleukin 6R, chemokine (C-C motif) ligand 2 (CCL2), chemokine (C-X-C motif) ligand 10 (CXCL10), oncostatin M, and vascular endothelial growth factor-A in CD14+ monocytes were quantified using reverse transcription-quantitative polymerase chain reaction. The results of the present study revealed that increased expression levels of CCL2 and CXCL10 were inversely correlated with the percentage of CD14+HLA-DRlow/- monocytes. This suggested that monocytes in RCC patients were immunologically suppressed, and that immunosuppression in RCC patients may be due, in part, to the dysfunction of circulating monocytes.