A new method for D-glucaric acid excretion measurement that is suitable for automated instruments.
A new method for D-glucaric acid excretion measurement that is suitable for automated instruments.
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一种适用于自动化仪器的 D-葡萄糖酸排泄测量新方法。
DOI：
10.1093/clinchem/34.11.2283
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发表时间：
1988
影响因子：
9.3
通讯作者：
A. Mondonico
中科院分区：
文献类型：
--
作者：
P. Mocarelli;Paolo Brambilla;L. Colombo;Alessandro Marocchi;C. Crespi;P. Tramacere;A. Mondonico
尿D-葡糖二酸（uGA）排泄是II型肝微粒体酶诱导的指标，间接揭示了某些药物和环境污染物可能的有机影响。然而，它的确定往往是繁琐的。我们提出了一种新的，快速的微量分析方法uGA测定β-葡萄糖醛酸酶（BG; EC 3.2.1.31）的活性抑制产生的uGA衍生的1，4-D-葡萄糖酸内酯进行测量。通过使用纯化BG，该方法适用于离心分析仪，允许每天测定超过100个样品。此外，该方法比基于BG抑制的其他酶促方法更准确地测量uGA。日内CV范围为7.9%-4.6%（uGA 31.55-121.31 mumol/L）;日间CV范围为11.5%-5.0%（uGA 26.09-124.10 mumol/L）。检出限为6.0 μ mol/L。标准曲线在10 ~ 200 μ mol/L范围内呈线性关系。平均分析回收率为100%。与Simmons等人（Clin Chim Acta 1974;51：47-51）的方法比较，相关性为r = 0.978，y = 1.40x-2.81。在369名健康人群样本（165名14岁以下）中建立了参考区间，以微摩尔/克肌酐表示的uGA在女性中高于女孩或男性。
Urinary excretion of D-glucaric acid (uGA) is an index of type II hepatic microsomal enzyme induction, indirectly revealing possible organic effects of some drugs and environmental pollutants. However, its determination is often cumbersome. We suggest a new, fast microanalytical method for uGA determination in which beta-glucuronidase (BG; EC 3.2.1.31) activity inhibition produced by uGA-derived 1,4-D-glucarolactone is measured. With use of purified BG, the method is suitable for centrifugal analyzers, allowing assay of greater than 100 samples per day. Moreover, the method measures uGA more accurately than other enzymatic methods based on BG inhibition. The within-day CV ranges from 7.9% to 4.6% (uGA 31.55-121.31 mumol/L); the between-day CV ranges from 11.5% to 5.0% (uGA 26.09-124.10 mumol/L). The detection limit is 6.0 mumol/L. The standard curve is linear from 10 to 200 mumol/L. Mean analytical recovery is 100%. Comparison with the method of Simmons et al. (Clin Chim Acta 1974;51:47-51) gave a correlation of r = 0.978, y = 1.40x-2.81. Reference intervals were established in a healthy population sample of 369 people (165 under 14 y), and uGA, expressed in micromoles per gram of creatinine, was higher in women than in girls or in males.