Downregulation of nucleophosmin expression inhibited proliferation and induced apoptosis in salivary gland adenoid cystic carcinoma
Downregulation of nucleophosmin expression inhibited proliferation and induced apoptosis in salivary gland adenoid cystic carcinoma
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DOI：
10.1111/jop.12482
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发表时间：
2017-03-01
影响因子：
3.3
通讯作者：
Huang, Zhiquan
中科院分区：
文献类型：
--
作者：
Li, Shihao;Zhang, Xinyu;Huang, Zhiquan
背景：本研究旨在探讨核磷蛋白（NPM 1）与患者临床特征的关系。材料与方法：采用免疫组化方法检测74例涎腺腺样囊性癌（SACC）和31例癌旁非癌上皮（NCEAC）中NPM 1的表达。利用RNA干扰技术沉默SACC细胞中NPM 1的表达。结果：NPM 1在SACC组织中核、胞浆均过表达，并与神经浸润和淋巴结转移密切相关。NPM 1的下调抑制SACC细胞的增殖并诱导凋亡。敲低NPM 1表达对SACC的耐药性、迁移或侵袭性没有影响。结论：NPM 1可能在SACC的肿瘤进展中发挥重要作用，是SACC的潜在生物标志物。
BACKGROUND: This study aimed to explore the relationship between nucleophosmin (NPM1) and patient clinical characteristics. Moreover, we investigated the effect of NPM1 in tumor proliferation and apoptosis of salivary gland adenoid cystic carcinoma (SACC).MATERIALS AND METHODS: NPM1 expression was examined in 74 specimens of SACC and 31 non-cancerous epithelium adjacent to carcinoma (NCEAC) by immunohistochemistry (IHC). RNA interference technology was used to silence NPM1 expression in SACC cells. We used transwell culture assay, cell counting kit-8 tests, and colony formation assay to test the proliferation, cisplatin resistance, migration, and invasiveness of SACC cells.RESULTS: The nuclear and cytoplasmic expression of NPM1 in SACC tissue was overexpressed and was tightly linked to perineural invasion and lymph node metastasis. The downregulation of NPM1 inhibited proliferation and induced apoptosis in SACC cells. Knockdown of NPM1 expression had no effect on chemoresistance migration, or invasiveness.CONCLUSIONS: NPM1 may play an important role in tumor progress in SACC and is a potential biomarker for SACC.