LEISHMANIA-DONOVANI - TITRATION OF ANTIBODIES TO THE FUCOSE-MANNOSE LIGAND AS AN AID IN DIAGNOSIS AND PROGNOSIS OF VISCERAL LEISHMANIASIS
LEISHMANIA-DONOVANI - TITRATION OF ANTIBODIES TO THE FUCOSE-MANNOSE LIGAND AS AN AID IN DIAGNOSIS AND PROGNOSIS OF VISCERAL LEISHMANIASIS
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DOI：
10.1016/0035-9203(95)90022-5
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发表时间：
1995-07-01
影响因子：
2.2
通讯作者：
BOROJEVIC, R
中科院分区：
文献类型：
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作者：
PALATNIKDESOUSA, CB;GOMES, EM;BOROJEVIC, R
岩藻糖-甘露糖配体（FML）是杜氏利什曼原虫前鞭毛体和无鞭毛体上的一种复杂糖蛋白组分。它以种特异性模式参与寄生虫与宿主巨噬细胞的相互作用。我们已经测试了其在内脏利什曼病（VL）的免疫诊断中的使用，在最近爆发的格兰德河做北，巴西东北部。酶联免疫吸附试验（ELISA）检测462份低浓度FML血清，敏感性为100%，特异性为96%。FML-ELISA鉴定出具有明显VL的患者（与正常血清相比P < 0.001）。它还可以识别流行地区的居民谁有早期或亚临床感染与潜在的严重临床疾病：超过20%的明显健康的受试者与FML阳性ELISA在接下来的10个月内发展明显VL。治疗期间所有患者FML-ELISA反应性均下降，经寄生虫学治疗后转为阴性。在感染其他利什曼原虫属的患者中没有观察到交叉反应，也没有观察到恰加斯病患者。对FML的抗体应答的测定可能有助于VL的诊断和识别没有明显疾病但具有发展为严重VL的高风险的患者。
The fucose-mannose ligand (FML) is a complex glycoprotein fraction present on promastigotes and amastigotes of Leishmania donovani. It participates in parasite interaction with host macrophages in a species-specific pattern. We have tested its use in immunodiagnosis of visceral leishmaniasis (VL) in a recent outbreak in Rio Grande do Norte, north-east Brazil. Enzyme-linked immunosorbent assay (ELISA) of low concentrations of FML in 462 sera showed 100% sensitivity and 96% specificity. The FML-ELISA identified patients with overt VL (P < 0.001 compared to normal sera). It could also identify inhabitants of the endemic area who had incipient or subclinical infection with potentially severe clinical disease: more than 20% of apparently healthy subjects with a positive ELISA for FML developed overt VL during the following 10 months. FML-ELISA reactivity decreased in all patients during treatment, and became negative after parasitological cure. No cross-reaction was observed in patients infected with other Leishmania species, nor in those with Chagas disease. Determination of antibody response to FML may be useful in diagnosis of VL and in identifying patients without overt disease but with a high risk of developing severe VL.