Impairment of Circulating CD4+CD25+GARP+ Regulatory T Cells in Patients with Acute Coronary Syndrome
Impairment of Circulating CD4+CD25+GARP+ Regulatory T Cells in Patients with Acute Coronary Syndrome
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DOI：
10.1159/000358639
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Zeng, Qiutang
中科院分区：
文献类型：
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作者：
Meng, Kai;Zhang, Wei;Zeng, Qiutang
背景：动脉粥样硬化(AS)是一种炎症性和免疫性疾病。调节性T细胞(Tregs)抑制T细胞的活化，在AS的发病过程中起到保护作用。然而，目前还缺乏Tregs的特异性标记。最近，糖蛋白A重复占优势(GARP)被发现是激活的Tregs的特异性标记，因此我们在本研究中使用GARP作为Tregs的特异性表面标记。方法：检测急性冠脉综合征(ACS)患者外周血中GARP(+)T细胞的频率及其相关的细胞因子和抑制功能，以评价急性冠脉综合征(ACS)患者GARP(+)Tregs是否下调。此外，我们比较了GARP和FOXP3的表达，FOXP3可能更敏感地作为急性冠脉综合征患者激活的Tregs的标志。结果：急性冠脉综合征患者外周血中CD4(+)、CD25(+)、GARP(+)Tregs明显减少。此外，与稳定型心绞痛(SA)和冠状动脉正常(NCA)患者相比，ACS患者的Tregs抑制功能和相关细胞因子水平也受到损害。此外，经TCR刺激后，急性冠脉综合征患者外周血单个核细胞(PBMC)中的CD4(+)CD25(+)GARP(+)Tregs明显减少。结论：这些发现表明ACS患者循环中的CD4(+)CD25(+)GARP(+)Tregs受损。因此，靶向GARP可能促进Tregs在急性冠脉综合征中的保护作用。版权所有(C)2014年S.Karger AG，巴塞尔
Background: Atherosclerosis (AS) is an inflammatory and immune disease. Regulatory T cells (Tregs) suppress the activation of T cells and have been shown to play a protective role during the pathogenesis of AS. However, specific markers for Tregs are lacking. Recently, glycoprotein A repetitions predominant (GARP) was discovered as a specific marker of activated Tregs, and we therefore utilized GARP as a specific surface marker for Tregs in the current study. Methods: To assess whether GARP(+) Tregs are downregulated in patients with acute coronary syndrome (ACS), we examined CD4(+)CD25(+)GARP(+) T cell frequencies as well as their associated cytokines and suppressive function. Additionally, we compared GARP expression to that of FOXP3, which may be more sensitive as a marker of activated Tregs in patients with ACS. Results: Patients with ACS demonstrated a significant decrease in circulating CD4(+)CD25(+)GARP(+) Tregs. Moreover, the suppressive function of Tregs and levels of related cytokines were also impaired in ACS patients compared to those with stable angina (SA) or normal coronary artery (NCA). Additionally, after TCR stimulation, peripheral blood mononuclear cells (PBMCs) from patients with ACS exhibited a decrease in CD4(+)CD25(+)GARP(+) Tregs. Conclusions: These finding indicate that circulating CD4(+)CD25(+)GARP(+) Tregs are impaired in patients withACS. Thus, targeting GARP may promote the protective function of Tregs in ACS. Copyright (C) 2014 S. Karger AG, Basel