THE PROTEIN-KINASE HOMOLOG STE20P IS REQUIRED TO LINK THE YEAST PHEROMONE RESPONSE G-PROTEIN BETA-GAMMA SUBUNITS TO DOWNSTREAM SIGNALING COMPONENTS
THE PROTEIN-KINASE HOMOLOG STE20P IS REQUIRED TO LINK THE YEAST PHEROMONE RESPONSE G-PROTEIN BETA-GAMMA SUBUNITS TO DOWNSTREAM SIGNALING COMPONENTS
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DOI：
10.1002/j.1460-2075.1992.tb05587.x
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发表时间：
1992-12-01
期刊：
影响因子：
11.4
通讯作者：
WHITEWAY, M
中科院分区：
文献类型：
--
作者：
LEBERER, E;DIGNARD, D;WHITEWAY, M
在酿酒酵母中，G 蛋白 betaamma 亚基已被证明可以触发信息素响应途径的下游事件。我们鉴定了一个新基因，命名为 STE20，它编码与蛋白激酶 C 序列相似的蛋白激酶同源物，该基因是将信息素信号从 G(betagamma) 传递到信号通路下游组件所必需的。该激酶的过量产生抑制了显性失活 G(β) 突变的交配缺陷，为与 G(β) 相互作用提供了遗传证据，并且上位性实验表明，该激酶在 G(betagamma) 之后或同一点发挥作用，但在信号通路中任何其他目前已识别的组件之前发挥作用。这指出了 G 蛋白介导的信号转导的潜在新机制，即通过 G(betagamma) 激活蛋白激酶。
In the yeast Saccharomyces cerevisiae the G-protein betagamma subunits have been shown to trigger downstream events of the pheromone response pathway. We have identified a new gene, designated STE20, which encodes a protein kinase homologue with sequence similarity to protein kinase C, which is required to transmit the pheromone signal from G(betagamma) to downstream components of the signalling pathway. Overproduction of the kinase suppresses the mating defect of dominant-negative G(beta) mutations providing genetic evidence for an interaction with G(beta), and epistasis experiments show that this kinase functions after or at the same point as G(betagamma), but before any of the other currently identified components of the signalling pathway. This points to a potentially new mechanism of G-protein mediated signal transduction, the activation of a protein kinase through G(betagamma).