TAXONOMY OF CLOSTRIDIA - RIBOSOMAL RIBONUCLEIC-ACID HOMOLOGIES AMONG SPECIES
TAXONOMY OF CLOSTRIDIA - RIBOSOMAL RIBONUCLEIC-ACID HOMOLOGIES AMONG SPECIES
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DOI：
10.1099/00221287-88-2-229
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发表时间：
1975-01-01
期刊：
影响因子：
--
通讯作者：
FRANCIS, BS
中科院分区：
文献类型：
--
作者：
JOHNSON, JL;FRANCIS, BS
对代表56种梭状芽胞杆菌的参考菌株进行了rRNA同源性测定。用氚标记的23个S核糖核酸进行竞争实验。大多数物种的DNA含有27%到28%的鸟嘌呤和胞嘧啶(%GC)。这些物种分为定义明确的rRNA同源组I和II，以及由不属于组I和组II的物种组成的第三组。DNA含量在41%到45%GC的物种组成第四组。其中30种与酪酸梭菌、产气荚膜梭菌、卡氏梭菌、产孢梭菌、新生梭菌的rRNA同源性在50%或以上的基础上归入第一组。巴氏杆菌。在同源性组I中描述了10个亚组。每个亚组中的物种要么与特定参考物种具有高度同源性，要么与所有参考生物具有相似的同源性模式。RRNA同源性组II中的11个物种具有69%或更高的同源性TOC。组I和组II中的物种具有20%到40%的组间同源性。组III中的6个种与组I和组II的同源性很低。当将其中5个种与第六个种--分枝冠藻进行比较时，同源性也很小。5个种的DNA中GC含量在41%到45%之间。Innocuum、蝶形隐杆藻(C.sphenoides.indolis)、巴氏隐杆藻(C.barkeriandC.RRNA同源性很小，在C。无毒和其他高%GC种，或与几个具有相似%GC DNA的芽孢杆菌。讨论了同源性结果与表型特征之间的相关性。
rRNA homologies have been determined on reference strains representing 56 species of Clostridium. Competition experiments using tritium-labelled 23 S rRNA were employed. The majority of the species had DNA with 27 to 28% guanine plus cytosine (% GC). These fell into rRNA homology groups I and II, which were well defined, and a third group which consisted of species which did not belong in groups I and II. Species whose DNA was 41 to 45% GC comprised a fourth group. Thirty species were placed into rRNA homology group I on the basis of having 50% or greater homology withClostridium butyricum, C. perfringens, C. carnis, C. sporogenes, C. novyiorC. pasteurianum.Ten subgroups were delineated in homology group I. Species in each subgroup either had high homology with a particular reference species or a similar pattern of homologies to all of the reference organisms. The eleven species in rRNA homology group II had 69% or greater homology toC. lituseburense.Species in groups I and II had intergroup homologies of 20 to 40%. The six species in group III had very low homologies with groups I and II. Negligible homology also resulted when five of the species were tested against the sixth,C. ramosum.The five species having DNA with 41 to 45% GC wereC. innocuum, C. sphenoides, C. indolis, C. barkeriandC. oroticum.Little rRNA homology was apparent betweenC. innocuumand the other high % GC species or with several Bacillus species having similar % GC DNA. Correlations between homology results and phenotypic characteristics are discussed.