PALM and STORM: What hides beyond the Rayleigh limit?
PALM and STORM: What hides beyond the Rayleigh limit?
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DOI：
10.1002/biot.200900024
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发表时间：
2009-06-01
影响因子：
4.7
通讯作者：
Mhlanga, Musa M.
中科院分区：
文献类型：
--
作者：
Henriques, Ricardo;Mhlanga, Musa M.
超分辨率成像允许荧光标记探针的成像分辨率仅为数十纳米，超过经典光学显微镜至少一个数量级。最新的进展，如光开关荧光团，高灵敏度显微镜和单粒子定位算法的发展，使超分辨率成像迅速进入更广泛的生命科学研究界。当我们迈出破译单个分子在其活细胞环境中的作用和行为的第一步时，一个新的研究机会世界正在召唤。在这里，我们讨论了一些最新的发展与这些技术和新兴领域，超分辨率将给予基本的新的“眼睛”的视线细胞生物学。
Super-resolution imaging allows the imaging of fluorescently labeled probes at a resolution of just tens of nanometers, surpassing classic light microscopy by at least one order of magnitude. Recent advances such as the development of photo-switchable fluorophores, high-sensitivity microscopes and single particle localization algorithms make super-resolution imaging rapidly accessible to the wider life sciences research community. As we take our first steps in deciphering the roles and behaviors of individual molecules inside their living cellular environment, a new world of research opportunities beckons. Here we discuss some of the latest developments achieved with these techniques and emerging areas where super-resolution will give fundamental new "eye" sight to cell biology.